尼古丁对大鼠正畸诱导牙根吸收的短期影响。
Short-term effects of nicotine on orthodontically induced root resorption in rats.
作者信息
Li Jing, Wang Xuxia, Li Na, Zhenga Dehua, Su Yuran, Zhang Jun
机构信息
a Student, Department of Orthodontics, School of Stomatology, Shandong University, Jinan, Shandong, China.
b Professor and Department Chair, Department of Oral and Maxillofacial Surgery, School of Stomatology, Shandong University, Jinan, Shandong, China.
出版信息
Angle Orthod. 2016 Mar;86(2):199-205. doi: 10.2319/101014-727.1. Epub 2015 Jun 17.
OBJECTIVE
To investigate the effect of nicotine exposure on root resorption in an in vivo rat model of orthodontic tooth movement (OTM), and its association with odontoclastogenesis and receptor activator of nuclear factor-kappa B ligand (RANKL) expression.
MATERIALS AND METHODS
Forty-eight 10-week-old male Wistar rats were divided into three groups. The negative control group was untreated. The left maxillary first molars in the nicotine-treated group and the positive control group received OTM with an initial force of 0.6 N in the mesial direction. Also, the nicotine-treated group received intraperitoneal injection of nicotine at 7 mg/kg per day. After 21 days, the rats were humanely killed. Eight rats from each group were randomly chosen for crater volume analysis by micro-computed tomography. For the remaining eight rats in each group, specimen slices were generated for histologic examination to determine the odontoclast number and the mean optical density value of RANKL.
RESULTS
The resorption volumes in the nicotine-treated group were significantly larger than those in the control groups. Also, the nicotine-treated group displayed significantly higher number of odontoclasts and elevated RANKL expression compared to the control groups.
CONCLUSIONS
In an in vivo rat model, nicotine exposure promotes odontoclastogenesis and RANKL expression, evoking aggravated root resorption during OTM.
目的
在正畸牙移动（OTM）的体内大鼠模型中研究尼古丁暴露对牙根吸收的影响，及其与破牙细胞生成和核因子κB受体激活剂配体（RANKL）表达的关系。
材料与方法
将48只10周龄雄性Wistar大鼠分为三组。阴性对照组不做处理。尼古丁处理组和阳性对照组的左上颌第一磨牙均以0.6 N的初始力向近中方向施加正畸力。此外，尼古丁处理组每天腹腔注射7 mg/kg的尼古丁。21天后，对大鼠实施安乐死。每组随机选取8只大鼠，通过微型计算机断层扫描分析牙槽骨吸收量。对于每组剩余的8只大鼠，制作标本切片进行组织学检查，以确定破牙细胞数量和RANKL的平均光密度值。
结果
尼古丁处理组的牙根吸收量显著大于对照组。此外，与对照组相比，尼古丁处理组的破牙细胞数量显著增多，RANKL表达升高。
结论
在体内大鼠模型中，尼古丁暴露促进破牙细胞生成和RANKL表达，导致正畸牙移动过程中牙根吸收加重。
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