Matić Petrović Sanja, Cimbaljević Milena, Radunović Milena, Kuzmanović Pfićer Jovana, Jotić Aleksandra, Pucar Ana
Department of Oral Medicine and Periodontology, School of Dental Medicine, University of Belgrade, Belgrade, Serbia.
Department of Microbiology and Immunology, School of Dental Medicine, University of Belgrade, Belgrade, Serbia.
Braz Oral Res. 2015;29. doi: 10.1590/1807-3107BOR-2015.vol29.0077. Epub 2015 Jun 16.
The purpose of this study was to detect Candida spp. on the tongue and in the subgingival sites in healthy and type 2 diabetes (T2D) patients with chronic periodontitis (CP), and to compare the accuracy of sampling methods. This study included 131 patients divided into four groups: healthy control (group A), nondiabetics + CP (Group B), diabetics with good metabolic control + CP (group C) and diabetics with poor glycoregulation + CP (Group D). Cotton swab samples from tongue and subgingival samples were obtained from each patient with help of sterile paper points and a sterile curette. Swab cultures were made on Sabouraud dextrose agar. The number of CFUs was counted. The sampling methods for subgingival plaque were compared by Receiving Operator Curve (ROC). The presence of Candida spp. on the tongue was statistically significant among groups (group D vs. others three groups: χ(2): p < 0.005 for each group). Positive findings of subgingival Candida spp. did not differ among the groups. There were no significant differences in the quantification of Candida spp., neither on the tongue, nor in the subgingival samples. 17.2% of diabetic patients revealed the presence of Candida spp. in the subgingival samples, with negative finding on tongue. There was a significant difference in the sampling methods for subgingival plaque (p = 0.000). Candida spp. is more prevalent on the tongue of diabetics. The sampling of subgingival plaque by a sterile curette is more accurate than with paper points. Subgingival plaque may represent a reservoir of commensals. It is necessary to standardize the sampling of subgingival plaque.
本研究旨在检测健康及患有慢性牙周炎（CP）的2型糖尿病（T2D）患者舌部及龈下部位的念珠菌属，并比较采样方法的准确性。本研究纳入131例患者，分为四组：健康对照组（A组）、非糖尿病+CP组（B组）、代谢控制良好的糖尿病+CP组（C组）和血糖调节不佳的糖尿病+CP组（D组）。借助无菌纸尖和无菌刮匙从每位患者获取舌部棉拭子样本和龈下样本。在沙氏葡萄糖琼脂上进行拭子培养。计算菌落形成单位（CFU）数量。通过接受者操作特征曲线（ROC）比较龈下菌斑的采样方法。各组间舌部念珠菌属的存在具有统计学意义（D组与其他三组比较：χ(2)：每组p<0.005）。龈下念珠菌属的阳性发现各组间无差异。念珠菌属的定量在舌部和龈下样本中均无显著差异。17.2%的糖尿病患者龈下样本中存在念珠菌属，而舌部检测为阴性。龈下菌斑的采样方法存在显著差异（p = 0.000）。念珠菌属在糖尿病患者舌部更为普遍。用无菌刮匙采集龈下菌斑比用纸尖更准确。龈下菌斑可能是共生菌的储存库。有必要规范龈下菌斑的采样。
