Cavana Paola, Peano Andrea, Petit Jean-Yanique, Tizzani Paolo, Perrot Sébastien, Bensignor Emmanuel, Guillot Jacques
Department of Parasitology, Mycology and Dermatology, Dynamic Research Group, Ecole Nationale Vétérinaire d'Alfort, UPE, 94704, Maisons-Alfort, France.
Department of Veterinary Sciences, University of Turin, Largo Paolo Braccini 2, 10095, Grugliasco, Italy.
Vet Dermatol. 2015 Aug;26(4):278-e61. doi: 10.1111/vde.12220. Epub 2015 Jun 16.
Wipes containing chlorhexidine and azole derivates have been recommended for veterinary use. No study has been published about their activity against Malassezia pachydermatis.
HYPOTHESIS/OBJECTIVES: To evaluate the in vivo and in vitro activity of wipes soaked in a chlorhexidine, climbazole and Tris-EDTA solution against Malassezia pachydermatis.
Five research colony shar-pei dogs.
Wipes were applied once daily onto the left axilla, left groin and perianal area (protocol A), and twice daily on the right axilla, right groin and umbilical region (protocol B) for 3 days. In vivo activity was evaluated by quantifying Malassezia colonies through contact plates on the selected body areas before and after wipe application. The activity of the solution in which the wipes were soaked was assessed in vitro by contact tests following the European Standard UNI EN 1275 guidelines.
Samples collected after wipe application showed a significant and rapid reduction of Malassezia yeast CFU. No significant difference in the Malassezia reduction was found between protocols A and B. In vitro assay showed 100% activity against Malassezia yeasts after a 15 min contact time with the wipe solution.
Wipes containing chlorhexidine, climbazole and Tris-EDTA substantially reduced the M. pachydermatis population on the skin of dogs. The results, although this was an uncontrolled study performed on a small number of dogs, suggest that these wipes may be useful for topical therapy of Malassezia dermatitis involving the lips, paws, perianal area and skin folds.
含氯己定和唑类衍生物的擦拭巾已被推荐用于兽医领域。尚未有关于其对厚皮马拉色菌活性的研究发表。
假设/目的：评估浸泡在氯己定、克霉唑和三乙醇胺 - 乙二胺四乙酸溶液中的擦拭巾对厚皮马拉色菌的体内和体外活性。
五只实验用沙皮犬。
擦拭巾按照方案A每天一次涂抹于左腋窝、左腹股沟和肛周区域，按照方案B每天两次涂抹于右腋窝、右腹股沟和脐部区域，持续3天。通过在擦拭巾涂抹前后对选定身体部位进行接触平板培养来定量马拉色菌菌落，从而评估体内活性。按照欧洲标准UNI EN 1275指南通过接触试验在体外评估浸泡擦拭巾的溶液活性。
擦拭巾涂抹后采集的样本显示马拉色菌酵母菌落形成单位显著且迅速减少。方案A和方案B之间在马拉色菌减少方面未发现显著差异。体外试验显示，与擦拭巾溶液接触15分钟后对马拉色菌酵母的活性为100%。
含氯己定、克霉唑和三乙醇胺 - 乙二胺四乙酸的擦拭巾可大幅减少犬皮肤表面的厚皮马拉色菌数量。尽管这是一项在少数犬只上进行的非对照研究，但其结果表明这些擦拭巾可能对涉及唇部、爪子、肛周区域和皮肤褶皱的马拉色菌性皮炎的局部治疗有用。
