Jang Chul Ho, Cho Yong Beom, Jang Yoon Seok, Kim Min Seong, Kim Geun Hyung
Department of Otolaryngology, Chonnam National University Medical School, Gwangju, South Korea.
Department of Otolaryngology, Chonnam National University Medical School, Gwangju, South Korea.
Int J Pediatr Otorhinolaryngol. 2015 Aug;79(8):1299-305. doi: 10.1016/j.ijporl.2015.05.037. Epub 2015 Jun 6.
In this study, we developed a biocompatible and biodegradable vancomycin (VM)-eluting electrospun poly(ɛ-caprolactone) (PCL)/polyethylene oxide (PEO)/VM/PCL nanofiber mat. The purpose of this study was to evaluate the efficacy of this nanofiber mat for preventing methicillin-resistant Staphylococcus aureus (MRSA) biofilm on the ossicular prostheses and otitis media.
Fabrication of VM eluting nanofiber mat was carried out using PCL and PEO. The release of VM was assessed by spectrophotometry in vitro. The antibacterial effect was evaluated by inhibition zone test and biofilm formation test in vitro. And prevention of MRSA infection by guinea pig was assessed by otoendoscopy, temporal bone CT and histopathologic examination in vivo.
VM released followed a triphasic pattern. VM eluting nanofiber mat prevented MRSA biofilm formation on the surface of ossicular prostheses regardless of materials in vitro, and MRSA otitis media in vivo. Otoendoscopy, temporal bone CT, and histopathologic findings revealed well ventilated middle ear.
From these results, VM eluting nanofiber mat may be a promising method for prevention of MRSA periprosthetic infection and biofilm formation.
在本研究中，我们制备了一种具有生物相容性和可生物降解性的载万古霉素（VM）的电纺聚己内酯（PCL）/聚环氧乙烷（PEO）/VM/PCL纳米纤维垫。本研究的目的是评估这种纳米纤维垫在预防听骨假体上耐甲氧西林金黄色葡萄球菌（MRSA）生物膜形成及中耳炎方面的效果。
使用PCL和PEO制备载VM的纳米纤维垫。通过分光光度法在体外评估VM的释放情况。通过体外抑菌圈试验和生物膜形成试验评估抗菌效果。通过耳内镜检查、颞骨CT和组织病理学检查在体内评估豚鼠预防MRSA感染的情况。
VM的释放呈三相模式。载VM的纳米纤维垫在体外可预防听骨假体表面MRSA生物膜的形成，而不考虑假体材料，在体内可预防MRSA中耳炎。耳内镜检查、颞骨CT和组织病理学结果显示中耳通气良好。
基于这些结果，载VM的纳米纤维垫可能是预防MRSA假体周围感染和生物膜形成的一种有前景的方法。
