Lim Khoon S, Ramaswamy Yogambha, Roberts Justine J, Alves Marie-Helene, Poole-Warren Laura A, Martens Penny J
Graduate School of Biomedical Engineering, University of New South Wales, Sydney, Australia, 2052.
Macromol Biosci. 2015 Oct;15(10):1423-32. doi: 10.1002/mabi.201500121. Epub 2015 Jun 11.
A photopolymerizable-tyraminated poly(vinyl alcohol) (PVA-Tyr) system that has the ability to covalently bind proteins in their native state was evaluated as a platform for cell encapsulation. However, a key hurdle to this system is the radicals generated during the cross-linking that can cause oxidative stress to the cells. This research hypothesized that incorporation of anti-oxidative proteins (sericin and gelatin) into PVA-Tyr gels would mitigate any toxicity caused by the radicals. The results showed that although incorporation of 1 wt% sericin promoted survival of the fibroblasts, both sericin and gelatin acted synergistically to facilitate long-term 3D cell function. The encapsulated cells formed clusters with deposition of laminin and collagen, as well as remaining metabolically active after 21 d.
一种具有在天然状态下共价结合蛋白质能力的可光聚合酪胺化聚乙烯醇（PVA-Tyr）体系被评估为细胞封装平台。然而，该体系的一个关键障碍是交联过程中产生的自由基，这些自由基会对细胞造成氧化应激。本研究假设将抗氧化蛋白（丝胶蛋白和明胶）掺入PVA-Tyr凝胶中可减轻自由基引起的任何毒性。结果表明，虽然掺入1 wt%的丝胶蛋白可促进成纤维细胞的存活，但丝胶蛋白和明胶协同作用以促进长期三维细胞功能。封装的细胞形成了含有层粘连蛋白和胶原蛋白沉积的簇，并且在21天后仍保持代谢活性。
