Kim Jeong Hwa, Lee Jae Kwon
Department of Biology Education, College of Education, Chungbuk National University, Chungbuk, 361-763, Republic of Korea.
College of Pharmacy, Chungbuk National University, Chungbuk-do, 361-763, Republic of Korea.
Arch Pharm Res. 2015 Nov;38(11):2042-8. doi: 10.1007/s12272-015-0624-5. Epub 2015 Jun 23.
Natural killer (NK) cells are capable of identifying and killing tumor cells as well as virus infected cells without pre-sensitization. NK cells express activating and inhibitory receptors, and can distinguish between normal and tumor cells. The present study was designed to demonstrate the importance of the expression level of NKG2D ligands on the Burkitt's lymphoma cell line, Raji, in enhancing NK cell cytolytic activity. Various flavonoids were used as stimulants to enhance the expression of NKG2D ligands. NK cell lysis activity against Raji was not changed by pre-treatment of Raji with luteolin, kaempferol, taxifolin and hesperetin. However, treatment of Raji with naringenin showed increased sensitivity to NK cell lysis than untreated control cells. The activity of naringenin was due to enhanced NKG2D ligand expression. These results provide evidence that narigenin's antitumor activity may be due to targeting of NKG2D ligand expression and suggests a possible immunotherapeutic role for cancer treatment.
自然杀伤（NK）细胞能够在无需预先致敏的情况下识别并杀死肿瘤细胞以及病毒感染细胞。NK细胞表达激活受体和抑制受体，并且能够区分正常细胞和肿瘤细胞。本研究旨在证明NKG2D配体在伯基特淋巴瘤细胞系Raji上的表达水平在增强NK细胞溶细胞活性方面的重要性。使用了各种黄酮类化合物作为刺激剂来增强NKG2D配体的表达。用木犀草素、山奈酚、紫杉叶素和橙皮素预处理Raji细胞，对NK细胞针对Raji的裂解活性没有影响。然而，用柚皮素处理Raji细胞后，其对NK细胞裂解的敏感性比未处理的对照细胞更高。柚皮素的这种活性归因于NKG2D配体表达的增强。这些结果提供了证据，表明柚皮素的抗肿瘤活性可能归因于对NKG2D配体表达的靶向作用，并提示其在癌症治疗中可能具有免疫治疗作用。
