Liang Fangfang, Jiang Shouqun, Mo Yi, Zhou Guilian, Yang Lin
Guangdong Public Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China .
Guangxi Research Center for Population and Family Planning, Nanning 530021, China .
Asian-Australas J Anim Sci. 2015 Aug;28(8):1194-201. doi: 10.5713/ajas.14.0924.
This study investigated the effect of oxidized soybean oil in the diet of young chickens on growth performance and intestinal oxidative stress, and indices of intestinal immune function. Corn-soybean-based diets containing 2% mixtures of fresh and oxidized soybean oil provided 6 levels (0.15, 1.01, 3.14, 4.95, 7.05, and 8.97 meqO2/kg) of peroxide value (POV) in the diets. Each dietary treatment, fed for 22 d, had 6 replicates, each containing 30 birds (n = 1,080). Increasing POV levels reduced average daily feed intake (ADFI) of the broilers during d 1 to 10, body weight and average daily gain at d 22 but did not affect overall ADFI. Concentrations of malondialdehyde (MDA) increased in plasma and jejunum as POV increased but total antioxidative capacity (T-AOC) declined in plasma and jejunum. Catalase (CAT) activity declined in plasma and jejunum as did plasma glutathione S-transferase (GST). Effects were apparent at POV exceeding 3.14 meqO2/kg for early ADFI and MDA in jejunum, and POV exceeding 1.01 meqO2/kg for CAT in plasma and jejunum, GST in plasma and T-AOC in jejunum. Relative jejunal abundance of nuclear factor kappa B (NF-κB) P50 and NF-κB P65 increased as dietary POV increased. Increasing POV levels reduced the jejunal concentrations of secretory immunoglobulin A and cluster of differentiation (CD) 4 and CD8 molecules with differences from controls apparent at dietary POV of 3.14 to 4.95 meqO2/kg. These findings indicated that growth performance, feed intake, and the local immune system of the small intestine were compromised by oxidative stress when young broilers were fed moderately oxidized soybean oil.
本研究调查了在幼鸡日粮中添加氧化大豆油对其生长性能、肠道氧化应激及肠道免疫功能指标的影响。以玉米 - 大豆为基础的日粮含有2%新鲜和氧化大豆油的混合物，提供了6个水平（0.15、1.01、3.14、4.95、7.05和8.97 meqO2/kg）的日粮过氧化值（POV）。每种日粮处理饲喂22天，有6个重复，每个重复包含30只鸡（n = 1,080）。POV水平升高会降低第1至10天肉鸡的平均日采食量（ADFI）、第22天的体重和平均日增重，但不影响总体ADFI。随着POV升高，血浆和空肠中丙二醛（MDA）浓度增加，而血浆和空肠中的总抗氧化能力（T - AOC）下降。血浆和空肠中的过氧化氢酶（CAT）活性下降，血浆谷胱甘肽S - 转移酶（GST）也下降。对于早期ADFI和空肠中的MDA，POV超过大于3.14 meqO2/kg时影响明显；对于血浆和空肠中的CAT、血浆中的GST以及空肠中的T - AOC，POV超过1.01 meqO2/kg时影响明显。随着日粮POV升高，空肠中核因子κB（NF - κB）P50和NF - κB P65的相对丰度增加。POV水平升高会降低空肠中分泌型免疫球蛋白A以及分化簇（CD）4和CD8分子的浓度，在日粮POV为3.14至4.95 meqO2/kg时与对照组的差异明显。这些结果表明，当给幼龄肉鸡饲喂中度氧化的大豆油时，氧化应激会损害其生长性能、采食量和小肠局部免疫系统。
