Pezzotti Giuseppe, Boffelli Marco, Miyamori Daisuke, Uemura Takeshi, Marunaka Yoshinori, Zhu Wenliang, Ikegaya Hiroshi
Kyoto Institute of Technology, Ceramic Physics Laboratory, Sakyo-ku, Matsugasaki, Kyoto 606-8126, JapanbLoma Linda University, Department of Orthopedic Research, Department of Orthopaedics, 11406 Loma Linda Drive, Suite 606 Loma Linda, California 92354, U.
Kyoto Institute of Technology, Ceramic Physics Laboratory, Sakyo-ku, Matsugasaki, Kyoto 606-8126, Japan.
J Biomed Opt. 2015 Jun;20(6):065008. doi: 10.1117/1.JBO.20.6.065008.
The possibility of examining soft tissues by Raman spectroscopy is challenged in an attempt to probe human age for the changes in biochemical composition of skin that accompany aging. We present a proof-of-concept report for explicating the biophysical links between vibrational characteristics and the specific compositional and chemical changes associated with aging. The actual existence of such links is then phenomenologically proved. In an attempt to foster the basics for a quantitative use of Raman spectroscopy in assessing aging from human skin samples, a precise spectral deconvolution is performed as a function of donors' ages on five cadaveric samples, which emphasizes the physical significance and the morphological modifications of the Raman bands. The outputs suggest the presence of spectral markers for age identification from skin samples. Some of them appeared as authentic "biological clocks" for the apparent exactness with which they are related to age. Our spectroscopic approach yields clear compositional information of protein folding and crystallization of lipid structures, which can lead to a precise identification of age from infants to adults. Once statistically validated, these parameters might be used to link vibrational aspects at the molecular scale for practical forensic purposes.
通过拉曼光谱检查软组织的可能性受到挑战，因为人们试图通过探测人体年龄来研究皮肤生化成分随衰老而发生的变化。我们提出了一份概念验证报告，以阐明振动特征与衰老相关的特定成分和化学变化之间的生物物理联系。然后从现象学上证明了这种联系的实际存在。为了为在评估人类皮肤样本衰老情况时定量使用拉曼光谱奠定基础，对五个尸体样本进行了精确的光谱去卷积，作为供体年龄的函数，这突出了拉曼谱带的物理意义和形态学改变。结果表明存在用于从皮肤样本中识别年龄的光谱标记。其中一些标记表现为真正的“生物钟”，因为它们与年龄的关联非常精确。我们的光谱方法能够清晰地提供蛋白质折叠和脂质结构结晶的成分信息，从而可以精确识别从婴儿到成年人的年龄。一旦经过统计验证，这些参数可用于在分子尺度上关联振动方面，以用于实际的法医目的。
