Blatchford P, Bentley-Hewitt K L, Stoklosinski H, McGhie T, Gearry R, Gibson G, Ansell J
1 The New Zealand Institute for Plant and Food Research Limited, Private Bag 11600, Palmerston North 4442, New Zealand.
2 Department of Food and Nutritional Sciences, The University of Reading, Reading RG6 6AP, United Kingdom.
Benef Microbes. 2015;6(6):829-39. doi: 10.3920/BM2015.0006. Epub 2015 Jun 30.
A new Actinidia chinensis gold-fleshed kiwifruit cultivar 'Zesy002' was tested to investigate whether it could positively modulate the composition of the human colonic microbiota. Digested Zesy002 kiwifruit was added to in vitro pH-controlled anaerobic batch fermenters that were inoculated with representative human faecal microbiota. Alterations to the gut microbial ecology were determined by 16S rRNA gene sequencing and metabolic end products were measured using gas chromatography and liquid chromatography - mass spectrometry. Results indicated a substantial shift in the composition of bacteria within the gut models caused by kiwifruit supplementation. Zesy002 supplemented microbiota had a significantly higher abundance of Bacteroides spp., Parabacteroides spp. and Bifidobacterium spp. after 48 h of fermentation compared with the start of the fermentation. Organic acids from kiwifruit were able to endure simulated gastrointestinal digestion and were detectable in the first 10 h of fermentation. The fermentable carbohydrates were converted to beneficial organic acids with a particular predilection for propionate production, corresponding with the rise in Bacteroides spp. and Parabacteroides spp. These results support the claim that Zesy002 kiwifruit non-digestible fractions can effect favourable changes to the human colonic microbial community and primary metabolites, and demonstrate a hitherto unknown effect of Zesy002 on colonic microbiota under in vitro conditions.
对一种新的中华猕猴桃金果肉猕猴桃品种“Zesy002”进行了测试，以研究其是否能积极调节人类结肠微生物群的组成。将经过消化的Zesy002猕猴桃添加到接种了代表性人类粪便微生物群的体外pH控制厌氧分批发酵罐中。通过16S rRNA基因测序确定肠道微生物生态的变化，并使用气相色谱和液相色谱 - 质谱法测量代谢终产物。结果表明，补充猕猴桃导致肠道模型内细菌组成发生显著变化。与发酵开始时相比，补充Zesy002的微生物群在发酵48小时后，拟杆菌属、副拟杆菌属和双歧杆菌属的丰度显著更高。来自猕猴桃的有机酸能够经受模拟胃肠道消化，并在发酵的前10小时内可检测到。可发酵碳水化合物转化为有益的有机酸，尤其倾向于产生丙酸，这与拟杆菌属和副拟杆菌属的增加相对应。这些结果支持了Zesy002猕猴桃不可消化部分可对人类结肠微生物群落和初级代谢产物产生有利变化的说法，并证明了在体外条件下Zesy002对结肠微生物群有迄今未知的影响。
