Manosroi Aranya, Akazawa Hiroyuki, Kitdamrongtham Worapong, Akihisa Toshihiro, Manosroi Worapaka, Manosroi Jiradej
Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand ; Manose Health and Beauty Research Center, Chiang Mai 50200, Thailand.
Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand ; Department of Biotechnology and Material Chemistry, Nihon University Junior College, Chiba 274-8501, Japan.
Evid Based Complement Alternat Med. 2015;2015:397181. doi: 10.1155/2015/397181. Epub 2015 Jun 7.
Thai/Lanna medicinal plant recipes have been used for the treatment of several diseases including liver cancer. In this study, methanolic extracts (MEs) of 23 plants were tested for antiproliferative activity on human hepatoma cell line (Hep G2) by the sulforhodamine B (SRB) assay. Nine MEs with potent antiproliferative activity (IC50 < 100 µg/mL) were obtained and further semipurified by liquid/liquid partition extraction. The semipurified fractions were tested for the antiproliferative and antioxidative activities. ME of Stemona collinsae and the semipurified extract and methanol-water fraction (MF) of Gloriosa superba gave the highest antiproliferative activity on HepG2 which were 4.79- and 50.07-fold cisplatin, respectively. The semipurified fractions showed an increased antiproliferative activity. MF of Caesalpinia sappan and HF of Senna alata showed the highest free radical scavenging and metal chelating activities, respectively. The compound in n-hexane fraction (HF) of Ventilago denticulata which showed an increase in antiproliferative activity comparing to its ME was isolated and identified as emodin. This study has demonstrated the potential of the ME from S. collinsae, MF from G. superba, and emodin isolated from V. denticulata, for further development as an antiliver cancer agent.
泰国/兰纳药用植物配方已被用于治疗包括肝癌在内的多种疾病。在本研究中，通过磺酰罗丹明B（SRB）测定法测试了23种植物的甲醇提取物（MEs）对人肝癌细胞系（Hep G2）的抗增殖活性。获得了9种具有强抗增殖活性（IC50 < 100 µg/mL）的MEs，并通过液-液分配萃取进一步进行半纯化。对半纯化级分进行了抗增殖和抗氧化活性测试。百部的ME以及大花嘉兰的半纯化提取物和甲醇-水级分（MF）对HepG2的抗增殖活性最高，分别是顺铂的4.79倍和50.07倍。半纯化级分显示出增强的抗增殖活性。苏木的MF和番泻叶的HF分别显示出最高的自由基清除和金属螯合活性。与Ventilago denticulata的ME相比，其正己烷级分（HF）中显示出抗增殖活性增加的化合物被分离并鉴定为大黄素。本研究证明了百部的ME、大花嘉兰的MF以及从Ventilago denticulata分离出的大黄素作为抗肝癌药物进一步开发的潜力。
