Manosroi J, Wilairat R, Kijjoa A, Manosroi A
a Faculty of Pharmacy, Chiang Mai University, Chiang Mai, Thailand.
b Pharmaceutical Cosmetic Raw Materials and Natural Products Research and Development Center (PCRNC), Institute for Science and Technology Research and Development (IST), Chiang Mai University, Chiang Mai, Thailand.
Pharm Biol. 2005;43(4):324-329. doi: 10.1080/13880200590951720.
Five Thai plants from the Guttiferae (Hypericum hookerianum. Wight & Arn, Garcinia speciosa. Wall, Garcinia xanthochymus. Hook f. ex. T. Anderson, Cratoxylum formosum. ssp. pruniflorum. (Kurz) Gogel, and Calophyllum polyanthum. Wall ex Choisy) and one from the Schisandraceae (Schisandra verruculosa.) were extracted by methanol and chloroform. The extracts were screened for free radical scavenging activity using the DPPH assay. All extracts showed a dose-dependent antioxidant activity. The most potent with the lowest IC values were observed in the methanol extracts from the wood of G. speciosa., which were 2.5- and 5.3-fold more potent than the two standard antioxidants, ascorbic acid and α.-tocopherol, respectively. Free radical scavenging activities ranging from moderate to high were observed in both methanol and chloroform extracts from H. hookerianum., C. formosum. ssp. pruniflorum., G. xanthochymus., S. verruculosa. and C. polyanthum.. The information from this study can explain the traditional use and the further development of these extracts into new pharmaceuticals.
对五株来自藤黄科的泰国植物（金丝桃、大叶藤黄、多花山竹子、台湾黄牛木和多花红厚壳）以及一株来自五味子科的植物（瘤枝五味子）进行甲醇和氯仿提取。采用DPPH法对提取物进行自由基清除活性筛选。所有提取物均表现出剂量依赖性抗氧化活性。在大叶藤黄木材的甲醇提取物中观察到最有效的提取物，其IC值最低，分别比两种标准抗氧化剂抗坏血酸和α-生育酚强2.5倍和5.3倍。在金丝桃、台湾黄牛木、多花山竹子、瘤枝五味子和多花红厚壳的甲醇和氯仿提取物中均观察到中等至高的自由基清除活性。本研究所得信息可以解释这些提取物的传统用途以及将其进一步开发成新药物的情况。
