Pomelova Vera G, Korenberg Edward I, Kuznetsova Tatiana I, Bychenkova Tatiana A, Bekman Natalya I, Osin Nikolay S
Laboratory of Molecular Diagnostics, Department of Biological Microassay, State Research Institute of Biological Engineering, Moscow, Russian Federation.
Department of Infections with Natural Focality, N. F. Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russian Federation.
PLoS One. 2015 Jul 6;10(7):e0130048. doi: 10.1371/journal.pone.0130048. eCollection 2015.
A single-tier immunoassay using the C6 peptide of VlsE (C6) from Borrelia burgdorferi sensu stricto (Bb) has been proposed as a potential alternative to conventional two-tier testing for the serologic diagnosis of Lyme disease in the United States and Europe.
To evaluate the performance of C6 peptide based multiplex Phosphorescence Analysis (PHOSPHAN) for the serologic confirmation of Lyme borreliosis (LB) in Russian patients.
Serum samples (n = 351) were collected from 146 patients with erythema migrans (EM); samples from 131 of these patients were taken several times prior to treatment and at different stages of recovery. The control group consisted of 197 healthy blood donors and 31 patients with other diseases, all from the same highly endemic region of Russia. All samples were analyzed by PHOSPHAN for IgM and IgG to Bb C6, recombinant OspC and VlsE proteins, and C6 peptides from B. garinii and B. afzelii.
IgM and IgG to Bb C6 were identified in 43 and 95 out of 131 patients (32.8 and 72.5%, respectively); seroconversion of IgM antibodies was observed in about half of the patients (51.2%), and of IgG antibodies, in almost all of them (88.4%). Additional detection of OspC-IgM and VlsE-IgM or IgG to C6 from B. garinii or B. afzelii did not contribute significantly to the overall sensitivity of the multiplex immunoassay.
The multiplex phosphorescence immunoassay is a promising method for simultaneously revealing the spectrum of antibodies to several Borrelia antigens. Detection of IgM and IgG to Bb C6 in the sera of EM patients provides effective serologic confirmation of LB and, with high probability, indicates an active infection process.
在美国和欧洲，一种使用狭义伯氏疏螺旋体（Bb）的VlsE（C6）的C6肽的单层免疫测定法已被提议作为莱姆病血清学诊断中传统双层检测的潜在替代方法。
评估基于C6肽的多重磷光分析（PHOSPHAN）在俄罗斯患者中血清学确诊莱姆病（LB）的性能。
从146例游走性红斑（EM）患者中采集血清样本（n = 351）；其中131例患者的样本在治疗前和恢复的不同阶段多次采集。对照组由197名健康献血者和31例患有其他疾病的患者组成，他们均来自俄罗斯同一高流行地区。所有样本均通过PHOSPHAN分析Bb C6、重组OspC和VlsE蛋白以及来自伽氏疏螺旋体和阿氏疏螺旋体的C6肽的IgM和IgG。
在131例患者中的43例（分别为32.8%）和95例（分别为72.5%）中检测到Bb C6的IgM和IgG；约一半的患者（51.2%）观察到IgM抗体血清转化，几乎所有患者（88.4%）观察到IgG抗体血清转化。额外检测伽氏疏螺旋体或阿氏疏螺旋体的OspC-IgM和VlsE-IgM或C6的IgG对多重免疫测定的总体敏感性没有显著贡献。
多重磷光免疫测定是一种有前途的方法，可同时揭示针对几种疏螺旋体抗原的抗体谱。在EM患者血清中检测Bb C6的IgM和IgG可有效血清学确诊LB，并且很可能表明存在活跃的感染过程。
