Miura T, Downey J M, Ooiwa H, Ogawa S, Adachi T, Noto T, Shizukuda Y, Iimura O
Second Department of Internal Medicine, Sapporo Medical College, Japan.
Jpn Heart J. 1989 Sep;30(5):695-708. doi: 10.1536/ihj.30.695.
The effect of a varying period of ischemia on the development of myocardial infarction was investigated in the rabbit. Radiomicrosphere measurements confirmed that the collateral blood flow is almost zero (0.02 +/- 0.01 ml/min/g) and without a significant transmural gradient in the rabbit heart (n = 15). A coronary branch of the left circumflex artery was occluded for 5, 10, 15, 30 or 60 min and then reperfused. The coronary branch was occluded permanently in another group of rabbits. Three days after the coronary occlusion, the infarct size was determined by hematoxylin-eosin and Mallory's staining and the ischemic zone size was determined by fluorescent particles. The results showed that the percentage of the ischemic zone infarcted (% infarction) vs the log of duration of ischemia yielded a sigmoid curve which could be linearized by probit analysis: Probits of % infarction = 3.05 x log (ischemic duration in minutes) + 0.33, r = 0.83, p less than 0.01. The regression equation indicated that 50% of the ischemic myocytes necrotized after 34 min of coronary artery occlusion. Unlike in the dog heart, the infarct of the rabbit heart first appears in the midmyocardium and then progresses towards both the endocardium and epicardium.
在兔身上研究了不同缺血时间对心肌梗死发展的影响。放射性微球测量证实，兔心脏的侧支血流几乎为零（0.02±0.01毫升/分钟/克），且无明显的透壁梯度（n = 15）。将左旋冠状动脉的一个分支闭塞5、10、15、30或60分钟，然后再灌注。在另一组兔中，将冠状动脉分支永久闭塞。冠状动脉闭塞三天后，通过苏木精-伊红染色和马洛里染色确定梗死面积，通过荧光颗粒确定缺血区面积。结果表明，缺血区梗死百分比（%梗死）与缺血持续时间的对数呈S形曲线，通过概率分析可将其线性化：%梗死的概率=3.05×缺血持续时间（分钟）的对数+0.33，r = 0.83 , p<0.01。回归方程表明，冠状动脉闭塞34分钟后，50%的缺血心肌细胞坏死。与犬心脏不同，兔心脏的梗死首先出现在心肌中层，然后向心内膜和心外膜发展。
