药用植物掌叶大黄（蓼科）的叶绿体全基因组序列
The complete chloroplast genome sequence of the medicinal plant Rheum palmatum L. (Polygonaceae).
作者信息
Fan Kai, Sun Xiao-Jie, Huang Min, Wang Xu-Mei
机构信息
a School of Pharmacy, Xi'an Jiaotong University , Xi'an , PR China.
出版信息
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Jul;27(4):2935-6. doi: 10.3109/19401736.2015.1060448. Epub 2015 Jul 8.
The complete chloroplast genome of the medicinal plant Rheum palmatum L. (Polygonaceae) has been reconstructed from the whole-genome Illumina sequencing data. The genome is 161 541 bp in length, and exhibits a typical quadripartite structure of the large (LSC, 86 518 bp) and small (SSC, 13 111 bp) single-copy regions, separated by a pair of inverted repeats (IRs, 30 956 bp each). The chloroplast genome contains 131 genes, including 84 protein-coding genes (78 PCG species), eight ribosomal RNA genes (four rRNA species) and 37 transfer RNA genes (28 tRNA species). Phylogenetic tree based on the maximum parsimony (MP) analysis of 65 chloroplast protein-coding genes for 13 taxa demonstrated a close relationship between R. palmatum and Fagopyrum esculentum subsp. ancestrale in Polygonaceae.
已利用全基因组Illumina测序数据重建了药用植物掌叶大黄（蓼科）的完整叶绿体基因组。该基因组长度为161541bp，呈现出典型的四分体结构，包括一个大的单拷贝区域（LSC，86518bp）和一个小的单拷贝区域（SSC，13111bp），它们被一对反向重复序列（IRs，各30956bp）隔开。叶绿体基因组包含131个基因，其中包括84个蛋白质编码基因（78种PCG）、8个核糖体RNA基因（4种rRNA）和37个转运RNA基因（28种tRNA）。基于对13个分类群的65个叶绿体蛋白质编码基因进行最大简约法（MP）分析构建的系统发育树表明，掌叶大黄与蓼科中的苦荞麦祖先亚种关系密切。
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