From the Departments of *Nuclear Medicine, †Endocrinology, ‡Radiodiagnosis, and §Transfusion Medicine, Postgraduate Institute of Medical Education and Research, Chandigarh.
Clin Nucl Med. 2015 Sep;40(9):697-700. doi: 10.1097/RLU.0000000000000850.
The study aims to carry out in vivo tracking of stem cells labeled with positron emission tomography (PET) tracer fluorine 18-fluorodeoxyglucose (F-FDG) and find adequate administration methods for these cells in diabetic patients.
Bone marrow aspirate was taken from the iliac crest of patients. Bone marrow mononuclear cells were separated and purified using centrifugation. These cells were then labeled with PET tracer F-FDG. The labeled stem cells were given in a total of 21 type 2 diabetes mellitus patients comprising 3 groups of 7 patients each. Cells were infused either in peripheral intravenous route or through the targeted routes into the superior pancreaticoduodenal artery and the splenic artery respectively. Biodistribution and quantification studies were carried out at 30 and 90 minutes of stem cell infusion.
Our results show that targeted approach resulted in homing and retention of stem cells in pancreas as compared with the intravenous route where no discernible homing of stem cells was there. Outside the pancreas, liver and spleen showed intense FDG labeled stem cell accumulation. In the intravenous group, lung fields showed retention of cells in the initial biodistribution study at 30 minutes with significant clearance in the delayed 90 minute image.
Infusion into the superior pancreaticoduodenal artery should be a preferred route than into the splenic artery as the former method resulted in better homing and retention of labeled stem cells. Homing is least likely to occur when the intravenous route is used.
本研究旨在对经正电子发射断层扫描（PET）示踪剂氟-18-氟代脱氧葡萄糖（F-FDG）标记的干细胞进行体内示踪，并为糖尿病患者找到这些细胞的合适给药方法。
从患者的髂嵴处采集骨髓抽吸物。使用离心法分离和纯化骨髓单核细胞。然后用 PET 示踪剂 F-FDG 对这些细胞进行标记。将标记的干细胞总共给予 21 名 2 型糖尿病患者，每组 7 名。细胞分别通过外周静脉途径或通过靶向途径注入胰十二指肠上动脉和脾动脉。在干细胞输注后 30 和 90 分钟进行生物分布和定量研究。
我们的结果表明，与静脉途径相比，靶向方法导致干细胞在胰腺中的归巢和保留，而静脉途径中没有明显的干细胞归巢。在胰腺外，肝脏和脾脏显示出强烈的 FDG 标记干细胞积累。在静脉组中，在初始生物分布研究中，肺部在 30 分钟时显示出细胞的保留，在延迟的 90 分钟图像中显著清除。
与注入脾动脉相比，将其注入胰十二指肠上动脉应该是一种更好的途径，因为前者导致标记的干细胞更好的归巢和保留。当使用静脉途径时，归巢最不可能发生。
