Qiu Kangqiang, Yu Bole, Huang Huaiyi, Zhang Pingyu, Huang Juanjuan, Zou Shanshan, Chen Yu, Ji Liangnian, Chao Hui
MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, P. R. China.
Sci Rep. 2015 Jul 17;5:10707. doi: 10.1038/srep10707.
Fluorescent tracking gene delivery could provide us with a better understanding of the critical steps in the transfection process. However, for in vivo tracking applications, a small diameter (<10 nm) is one of the rigorous requirements for tracking vectors. Herein, we have demonstrated a new paradigm for two-photon tracking gene delivery based on a dendritic nano-sized hexanuclear ruthenium(II) polypyridyl complex. Because this metallodendrimer has a multivalent periphery, the complex, which is 6.1 nm, showed high stability and excellent dispersibility and could stepwise condense DNA in vitro. With the outstanding photochemical properties of Ru(II) polypyridyl, this complex could track gene delivery in vivo using one- and two-photon imaging.
荧光追踪基因递送能够让我们更好地理解转染过程中的关键步骤。然而，对于体内追踪应用而言，小直径（<10 nm）是追踪载体的严格要求之一。在此，我们展示了一种基于树枝状纳米尺寸的六核钌（II）多吡啶配合物的双光子追踪基因递送新范例。由于这种金属树枝状大分子具有多价外围，该6.1 nm的配合物表现出高稳定性和优异的分散性，并且能够在体外逐步凝聚DNA。凭借钌（II）多吡啶出色的光化学性质，这种配合物能够利用单光子和双光子成像在体内追踪基因递送。
