RuNH2@AuNPs 作为用于活细胞和组织中硫醇的双光子荧光探针。
RuNH2@AuNPs as two-photon luminescent probes for thiols in living cells and tissues.
机构信息
MOE Laboratory of Bioinorganic and Synthetic Chemistry, State Key Laboratory of Optoelectronic Materials and Technologies, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, PR China.
MOE Laboratory of Bioinorganic and Synthetic Chemistry, State Key Laboratory of Optoelectronic Materials and Technologies, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, PR China.
出版信息
Biomaterials. 2014 Oct;35(32):9003-11. doi: 10.1016/j.biomaterials.2014.07.021. Epub 2014 Aug 4.
Two-photon luminescent sensors have emerged as promising molecular tools for imaging biomolecules in living systems. Here, we present hybrid gold nanocomposites RuNH2@AuNPs as luminescence off-on probes in response to thiols, which can replace the Ru(II) complexes on the surfaces of the AuNPs to release the luminophore RuNH2. The liberated Ru(II) complexes exhibit strong two-photon luminescence and a large two-photon absorption cross section by using the two-photon excitation wavelength at 800 nm. Furthermore, the probe responses toward thiols with high selectivity and insensitivity to pH over the biologically relevant pH range. This two-photon probe can visualize biological thiols levels in live cells as well as in living mouse tissues at depths of 80-170 μm by two-photon microscopy.
双光子发光传感器已成为在活系统中成像生物分子的有前途的分子工具。在这里，我们提出了混合金纳米复合材料 RuNH2@AuNPs 作为对硫醇的发光关闭-开启探针，其可以替代 AuNPs 表面上的 Ru(II) 配合物以释放发光体 RuNH2。通过使用 800nm 的双光子激发波长，释放的 Ru(II) 配合物表现出强的双光子发光和大的双光子吸收截面。此外，该探针对具有高选择性和对生物相关 pH 范围的 pH 不敏感的硫醇作出响应。这种双光子探针可以通过双光子显微镜在 80-170μm 的深度可视化活细胞以及活体小鼠组织中的生物硫醇水平。
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