Wu Yue-Hong, Xu Lin, Zhou Peng, Wang Chun-Sheng, Oren Aharon, Xu Xue-Wei
Laboratory of Marine Ecosystem and Biogeochemistry, State Oceanic Administration, State Oceanic Administration, Hangzhou 310012, PR China.
Department of Plant and Environmental Sciences, Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Givat Ram, Jerusalem 91904, Israel.
Int J Syst Evol Microbiol. 2015 Oct;65(10):3645-3651. doi: 10.1099/ijsem.0.000469.
A Gram-stain-negative, motile, aerobic bacterial strain, designated 22DY15T, was isolated from a deep-sea sediment sample collected from a hydrothermal vent field located in the East Pacific Rise. The isolate was a short rod with a single flagellum and was positive for catalase and oxidase activities. Q-10 was the predominant respiratory quinone. The major polar lipids consisted of phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphoglycolipid, one aminolipid and three unidentified phospholipids. The principal fatty acid (>70 %) was C18 : 1ω7c. The genomic DNA G+C content was 64.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 22DY15T represents a distinct lineage within the family Rhodobacteraceae. The closest relatives were species of the genera Aliiroseovarius (93.3–96.0 % 16S rRNA gene sequence similarity), Sulfitobacter (94.0–96.0 %) and Loktanella (92.0–95.9 %). Differential phenotypic properties, together with phylogenetic and genetic distinctiveness, revealed that strain 22DY15T could be differentiated from its most closely related genera. Therefore, it is proposed that strain 22DY15T represents a novel species in a new genus of the family Rhodobacteraceae, for which the name Brevirhabdus pacifica gen. nov., sp. nov. is proposed. The type strain of the type species is 22DY15T ( = JCM 19489T = DSM 27767T = CGMCC 1.12416T = MCCC 1K00276T).
从东太平洋海隆热液喷口区域采集的深海沉积物样本中分离出一株革兰氏阴性、具运动性、好氧的细菌菌株，命名为22DY15T。该分离株为短杆状，具单根鞭毛，过氧化氢酶和氧化酶活性呈阳性。泛醌Q-10是主要的呼吸醌。主要极性脂包括磷脂酰甘油、磷脂酰乙醇胺、磷脂酰胆碱、磷酸糖脂、一种氨基脂和三种未鉴定的磷脂。主要脂肪酸（>70%）为C18:1ω7c。基因组DNA的G+C含量为64.3 mol%。基于16S rRNA基因序列的系统发育分析表明，菌株22DY15T代表红杆菌科内一个独特的谱系。其最接近的亲缘种是Aliiroseovarius属（16S rRNA基因序列相似性为93.3–96.0%）、硫杆菌属（94.0–96.0%）和洛氏菌属（92.0–95.9%）的物种。不同的表型特征，以及系统发育和遗传独特性，表明菌株22DY15T可与其最密切相关的属区分开来。因此，建议将菌株22DY15T代表红杆菌科一个新属中的一个新物种，为此提出新属名为Brevirhabdus pacifica gen. nov., sp. nov.。模式种的模式菌株为22DY15T（=JCM 19489T=DSM 27767T=CGMCC 1.12416T=MCCC 1K00276T）。
