Zhang Yan-Jiao, Liu Xu-Feng, Kuang Bao-Zhi, Zhang Xi-Ying, Zhou Ming-Yang, Chen Shiyong
Shandong Province Key Laboratory of Applied Mycology, School of Life Sciences, Qingdao Agricultural University, Qingdao 266109, PR China.
State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, PR China.
Int J Syst Evol Microbiol. 2018 May;68(5):1702-1706. doi: 10.1099/ijsem.0.002728. Epub 2018 Mar 26.
A novel Gram-stain-negative bacterium, designated strain CY02, was isolated from sediment of the Yellow Sea. Cells of CY02 were aerobic, coccus or short rods. Growth occurred at 5-42 °C (optimum, 35 °C), pH 6-10 (optimum, 8.0) and 0.5-9.0 % NaCl (optimum, 1.5-3.0 %). Phylogenetic analysis of 16S rRNA gene sequences revealed that CY02 was a member of the family Rhodobacteraceae and exhibited less than 95 % sequence similarities with the closely related type strains of the family Rhodobacteraceae. The genomic DNA G+C content of CY02 was 57.5 mol%. The major respiratory quinone was ubiquinone-10 (Q-10). The polar lipids consisted of phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, three unidentified lipids, one unidentified phospholipid and one unidentified aminolipid. The predominant cellular fatty acids were C18 : 1ω7c (57.6 %), 11-methyl C18 : 1ω7c (22.8 %) and C16 : 0 (10.6 %). Based on the results of morphological, physiological, biochemical, chemotaxonomic, genotypic and phylogenetic analyses, strain CY02 represents a novel species of a novel genus of the family Rhodobacteraceae, for which the name Neptunicoccus sediminis gen. nov., sp. nov. is proposed. The type strain of Neptunicoccus sediminis is CY02 (=CCTCC AB 2015430=KCTC 42985=NBRC 111872=MCCC 1K03518).
从黄海沉积物中分离出一株新型革兰氏阴性菌，命名为CY02菌株。CY02菌株的细胞为需氧型，呈球菌或短杆状。该菌在5-42°C（最适温度35°C）、pH值6-10（最适pH值8.0）和0.5-9.0% NaCl（最适盐度1.5-3.0%）条件下生长。16S rRNA基因序列的系统发育分析表明，CY02菌株属于红杆菌科，与红杆菌科亲缘关系较近的模式菌株的序列相似性低于95%。CY02菌株的基因组DNA G+C含量为57.5 mol%。主要呼吸醌为泛醌-10（Q-10）。极性脂质包括磷脂酰胆碱、磷脂酰甘油、磷脂酰乙醇胺、双磷脂酰甘油、三种未鉴定的脂质、一种未鉴定的磷脂和一种未鉴定的氨基脂质。主要的细胞脂肪酸为C18:1ω7c（57.6%）、11-甲基C18:1ω7c（22.8%）和C16:0（10.6%）。基于形态学、生理学、生化、化学分类学、基因型和系统发育分析结果，CY02菌株代表红杆菌科一个新属的一个新物种，为此提出新属名为海王星球菌属（Neptunicoccus gen. nov.），新种名为黄海海王星球菌（Neptunicoccus sediminis sp. nov.）。黄海海王星球菌的模式菌株为CY02（=CCTCC AB 2015430=KCTC 42985=NBRC 111872=MCCC 1K03518）。
