KAUST Global Collaborative Research Program, Division of Life Science, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong.
Institute of Marine Science and Technology, Yangzhou University. No. 196, West Huayang Street, Yangzhou City, Jiangsu Province, PR China.
Int J Syst Evol Microbiol. 2013 Mar;63(Pt 3):1007-1012. doi: 10.1099/ijs.0.041525-0. Epub 2012 Jun 8.
A slow-growing, strictly aerobic, Gram-negative, coccus bacterial strain, designated KAUST100406-0324(T), was isolated from sea-floor sediment collected from the Red Sea, Saudi Arabia. The catalase- and oxidase-positive strain was non-sporulating and only slightly halophilic. Optimum growth occurred at 20-25 °C and at pH values ranging from 7.0 to 8.0. The major cellular fatty acids of the strain were unsaturated C18 : 1ω6c and/or C18 : 1ω7c, C18 : 1ω7c 11-methyl and C16 : 1ω7c and/or C16 : 1ω6c. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine and two unidentified phospholipids. Ubiquinone 10 was the predominant lipoquinone. The DNA G+C content of strain KAUST100406-0324(T) was 64.0 mol%. Phylogenetic analysis of 16S rRNA gene sequences revealed that the novel strain belonged to the family Rhodobacteraceae of the class Alphaproteobacteria but formed a distinct evolutionary lineage from other bacterial species with validly published names. The 16S rRNA gene sequence of the novel strain was distantly related, but formed a monophyletic cluster with, those of bacteria from two moderately halophilic genera, Hwanghaeicola and Maribius. The similarity of the sequence between the novel strain KAUST100406-0324(T) and the type strains Hwanghaeicola aestuarii Y26(T) (accession number FJ230842), Maribius pelagius B5-6(T) (DQ514326) and Maribius salinus CL-SP27(T) (AY906863) were 94.5 %, 95.2 % and 95.3 %, respectively. Based on the physiological, phylogenetic and chemotaxonomic characteristics presented in this study, we propose that this strain represents a novel species of a new genus in the family Rhodobacteraceae, for which the name of Profundibacterium mesophilum gen. nov., sp. nov. was proposed, with KAUST100406-0324(T) ( = JCM 17872(T) = NRRL B-59665(T)) as the type strain.
一株慢生长、严格需氧、革兰氏阴性、球菌的细菌菌株，被命名为KAUST100406-0324(T)，从沙特阿拉伯红海海底沉积物中分离得到。该菌株为过氧化氢酶和氧化酶阳性，不产芽孢且仅轻度嗜盐。最适生长温度为 20-25°C，pH 值范围为 7.0-8.0。该菌株的主要细胞脂肪酸为不饱和 C18 : 1ω6c 和/或 C18 : 1ω7c、C18 : 1ω7c 11-甲基和 C16 : 1ω7c 和/或 C16 : 1ω6c。主要极性脂为磷脂酰甘油、磷脂酰乙醇胺和两种未鉴定的磷脂。泛醌 10 是主要的脂醌。菌株 KAUST100406-0324(T)的 DNA G+C 含量为 64.0 mol%。16S rRNA 基因序列的系统发育分析表明，该新菌株属于α变形菌纲红杆菌科，但与其他具有有效发表名称的细菌物种形成了一个独特的进化谱系。新菌株的 16S rRNA 基因序列与两种中度嗜盐属的细菌，黄海杆菌和海洋玛利贝斯菌的序列关系较远，但形成了一个单系聚类。新菌株 KAUST100406-0324(T)与黄海杆菌河口亚种 Y26(T)（登录号 FJ230842）、海洋玛利贝斯菌 B5-6(T)（DQ514326）和海洋玛利贝斯菌盐生亚种 CL-SP27(T)（AY906863）的序列相似性分别为 94.5%、95.2%和 95.3%。基于本研究中呈现的生理、系统发育和化学分类特征，我们建议该菌株代表红杆菌科一个新属的一个新种，命名为嗜冷深海杆菌属，新种，其模式菌株为 Profundibacterium mesophilum gen. nov.，sp. nov.，KAUST100406-0324(T)（=JCM 17872(T)=NRRL B-59665(T)）为其模式菌株。
