Shanghai Engineering Research Center of Hadal Science and Technology, College of Marine Sciences, Shanghai Ocean University, Shanghai, China.
National Engineering Research Center for Oceanic Fisheries, Shanghai Ocean University, Shanghai, China.
Microbiologyopen. 2020 Feb;9(2):e966. doi: 10.1002/mbo3.966. Epub 2019 Nov 19.
A novel piezophilic alphaproteobacterium, strain D4M1 , was isolated from deep seawater of the Mariana Trench. 16S rRNA gene analysis showed that strain D4M1 was most closely related to Oceanicella actignis PRQ-67 (94.2%), Oceanibium sediminis O448 (94.2%), and Thioclava electrotropha ElOx9 (94.1%). Phylogenetic analyses based on both 16S rRNA gene and genome sequences showed that strain D4M1 formed an independent monophyletic branch paralleled with the genus Oceanicella in the family Rhodobacteraceae. Cells were Gram-stain-negative, aerobic short rods, and grew optimally at 37°C, pH 6.5, and 3.0% (w/v) NaCl. Strain D4M1 was piezophilic with the optimum pressure of 10 MPa. The principal fatty acids were C ω7c/C ω6c and C , major respiratory quinone was ubiquinone-10, and predominant polar lipids were phosphatidylglycerol, phosphatidylethanolamine, and an unidentified aminophospholipid. The complete genome contained 5,468,583-bp with a G + C content of 70.2 mol% and contained 4,855 protein-coding genes and 78 RNA genes. Genomic analysis revealed abundant clues on bacterial high-pressure adaptation and piezophilic lifestyle. The combined evidence shows that strain D4M1 represents a novel species of a novel genus in the family Rhodobacteraceae, for which the name Paraoceanicella profunda gen. nov., sp. nov. is proposed (type strain D4M1 = MCCC 1K03820 = KCTC 72285 ).
一株嗜压海洋 α 变形菌 D4M1 ，从马里亚纳海沟深海海水中分离得到。16S rRNA 基因分析表明，菌株 D4M1 与海洋游动杆菌 PRQ-67（94.2%）、海洋希瓦氏菌 O448（94.2%）和电硫代盐杆菌 ElOx9（94.1%）最为密切相关。基于 16S rRNA 基因和基因组序列的系统发育分析表明，菌株 D4M1 与海洋游动杆菌属形成一个独立的单系分支，与海洋游动杆菌属在红杆菌科中平行。细胞革兰氏阴性，好氧短杆菌，最适生长温度为 37°C，pH6.5，3.0%（w/v）NaCl。菌株 D4M1 是嗜压菌，最适压力为 10 MPa。主要脂肪酸为 C ω7c/C ω6c 和 C ，主要呼吸醌为泛醌-10，主要极性脂为磷脂酰甘油、磷脂酰乙醇胺和一种未鉴定的氨基磷脂。完整基因组包含 5468583-bp，G+C 含量为 70.2 mol%，包含 4855 个蛋白编码基因和 78 个 RNA 基因。基因组分析揭示了细菌高压适应和嗜压生活方式的丰富线索。综合证据表明，菌株 D4M1 代表红杆菌科一个新属的一个新种，建议将其命名为深海拟海洋菌属（Paraoceanicella profunda gen. nov.，sp. nov.），模式菌株为 D4M1 （=MCCC 1K03820 =KCTC 72285 ）。
