College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China.
College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China.
Biosens Bioelectron. 2014 Oct 15;60:64-70. doi: 10.1016/j.bios.2014.04.006. Epub 2014 Apr 16.
An ultrasensitive nanosensor based on fluorescence resonance energy transfer (FRET) between biocompatible graphene quantum dots and carbon nanotubes for DNA detection was reported. We take advantage of good biocompatibility and strong fluorescence of graphene quantum dots, base pairing specificity of DNA and unique fluorescence resonance energy transfer between graphene quantum dots and carbon nanotubes to achieve the analysis of low concentrations of DNA. Graphene quantum dots with high quantum yield up to 0.20 were prepared and served as the fluorophore of DNA probe. FRET process between graphene quantum dots-labeled probe and oxidized carbon nanotubes is easily achieved due to their efficient self-assembly through specific π-π interaction. This nanosensor can distinguish complementary and mismatched nucleic acid sequences with high sensitivity and good reproducibility. The detection method based on this nanosensor possesses a broad linear span of up to 133.0 nM and ultralow detection limit of 0.4 nM. The constructed nanosensor is expected to be highly biocompatible because of all its components with excellent biocompatibility.
本文报道了一种基于生物相容性石墨烯量子点与碳纳米管之间荧光共振能量转移（FRET）的超灵敏纳米传感器，用于 DNA 检测。我们利用石墨烯量子点良好的生物相容性和强荧光性、DNA 的碱基配对特异性以及石墨烯量子点与碳纳米管之间独特的荧光共振能量转移，实现了低浓度 DNA 的分析。制备了量子产率高达 0.20 的高量子产率石墨烯量子点，并将其用作 DNA 探针的荧光团。由于石墨烯量子点标记的探针通过特定的π-π相互作用能够有效地自组装，因此很容易实现 FRET 过程。该纳米传感器具有高灵敏度和良好重现性，能够区分互补和错配的核酸序列。基于该纳米传感器的检测方法具有很宽的线性范围（可达 133.0 nM）和超低的检测限（低至 0.4 nM）。由于其所有组件均具有优异的生物相容性，因此所构建的纳米传感器有望具有很高的生物相容性。
