用于诊断肺炎球菌性胸部感染的对流免疫电泳
Counter-current immunoelectrophoresis for the diagnosis of pneumococcal chest infection.
作者信息
El-Refaie M, Dulake C
出版信息
J Clin Pathol. 1975 Oct;28(10):801-6. doi: 10.1136/jcp.28.10.801.
Abstract
Counter-current immunoelectrohporesis is a rapid sensitive method for detecting pneumococcal capsular antigens in sputum. A result can be obtained within 45 minutes. The optimum conditions for performing the test are given. Counter-current immunoelectrophoresis works with all the 33 pneumococcal antigens tested except type 14. Better precipitin lines are obtained when the gel-support is acid (pH 6-6) than when it is alkaline (pH 8-6). Omniserum is as effective as group-specific sera for the identification of pneumococcal capsular antigens. The findings suggest that pneumococcal infection was present in 44% of 300 unselected suspected chest infections. Culture for pneumococci was positive in only 15% of these cases. The clinical importance of these findings is still being studied but our experience has shown that patients with chest infections should have effective antipneumococcal antibiotics as part of their regimen and that the laboratory diagnosis may be made quickly and accurately by counter-current immunoelectrophoresis.
摘要
对流免疫电泳是一种检测痰液中肺炎球菌荚膜抗原的快速灵敏方法。45分钟内即可得出结果。文中给出了进行该检测的最佳条件。除14型外，对流免疫电泳可用于检测所有33种肺炎球菌抗原。凝胶支持物呈酸性（pH 6 - 6）时比呈碱性（pH 8 - 6）时能获得更好的沉淀线。全效血清在鉴定肺炎球菌荚膜抗原方面与群特异性血清效果相同。研究结果表明，在300例未经挑选的疑似胸部感染病例中，44%存在肺炎球菌感染。这些病例中肺炎球菌培养仅15%呈阳性。这些发现的临床重要性仍在研究中，但我们的经验表明，胸部感染患者的治疗方案中应包含有效的抗肺炎球菌抗生素，并且通过对流免疫电泳可快速准确地进行实验室诊断。
相似文献
1
Counter-current immunoelectrophoresis for the diagnosis of pneumococcal chest infection.用于诊断肺炎球菌性胸部感染的对流免疫电泳
J Clin Pathol. 1975 Oct;28(10):801-6. doi: 10.1136/jcp.28.10.801.
2
Routine use of counterimmunoelectrophoresis for the detection of pneumococcal antigen in sputum.常规使用对流免疫电泳法检测痰液中的肺炎球菌抗原。
Med Microbiol Immunol. 1986;175(4):241-9. doi: 10.1007/BF02123732.
3
The value of immunoelectroosmophoresis (IEOP) for etiological diagnosis of acute respiratory tract infections due to pneumococci and Mycoplasma pneumoniae.免疫电渗电泳（IEOP）在肺炎球菌和肺炎支原体所致急性呼吸道感染病因诊断中的价值。
Scand J Infect Dis. 1978;10(3):173-6. doi: 10.3109/inf.1978.10.issue-3.03.
4
Use of Counter and rocket immunoelectrophoresis in acute respiratory infections due to Streptococcus pneumoniae.对流免疫电泳和火箭免疫电泳在肺炎链球菌所致急性呼吸道感染中的应用
J Clin Pathol. 1976 Mar;29(3):187-90. doi: 10.1136/jcp.29.3.187.
5
Countercurrent immunoelectrophoresis: improved detection of pneumococcal capsular antigens in sputum by incorporation of a carboxylated derivative of phenyl boronic acid.对流免疫电泳：通过加入苯基硼酸的羧化衍生物改进痰液中肺炎球菌荚膜抗原的检测。
J Clin Pathol. 1979 Sep;32(9):960-2. doi: 10.1136/jcp.32.9.960.
6
Detection of pneumolysin in sputum.痰液中肺炎溶血素的检测
J Med Microbiol. 1999 Sep;48(9):863-866. doi: 10.1099/00222615-48-9-863.
7
Counterimmunoelectrophoresis of pneumococcal antigens:improved sensitivity for the detection of types VII and XIV.
J Clin Microbiol. 1975 Dec;2(6):510-5. doi: 10.1128/jcm.2.6.510-515.1975.
8
Sputum counterimmunoelectrophoresis in the diagnosis of pneumococcal pneumonia.痰液对流免疫电泳在肺炎球菌肺炎诊断中的应用
Am Rev Respir Dis. 1979 Mar;119(3):345-8. doi: 10.1164/arrd.1979.119.3.345.
9
The diagnosis of pneumococcal chest infection by counter-current-immunoelectrophoresis.通过对流免疫电泳诊断肺炎球菌性胸部感染。
J Appl Bacteriol. 1980 Dec;49(3):471-8. doi: 10.1111/j.1365-2672.1980.tb04722.x.
10
Comparison of counterimmunoelectrophoresis and latex particle agglutination in the detection of Streptococcus pneumoniae in blood cultures.血培养中肺炎链球菌检测的对流免疫电泳与乳胶颗粒凝集试验比较
J Hosp Infect. 1983 Jun;4(2):165-71. doi: 10.1016/0195-6701(83)90046-4.
引用本文的文献
1
Development and Validation of a Sensitive and Robust Multiplex Antigen Capture Assay to Quantify Streptococcus pneumoniae Serotype-Specific Capsular Polysaccharides in Urine.开发并验证了一种灵敏且稳健的多重抗原捕获检测方法，用于定量尿液中的肺炎链球菌血清型特异性荚膜多糖。
mSphere. 2022 Aug 31;7(4):e0011422. doi: 10.1128/msphere.00114-22. Epub 2022 Aug 1.
2
Validation of an immunodiagnostic assay for detection of 13 Streptococcus pneumoniae serotype-specific polysaccharides in human urine.用于检测人尿中13种肺炎链球菌血清型特异性多糖的免疫诊断检测方法的验证
Clin Vaccine Immunol. 2012 Aug;19(8):1131-41. doi: 10.1128/CVI.00064-12. Epub 2012 Jun 6.
3
Oropharyngeal production of pneumococcal capsular antigen and the potential for contamination of expectorated sputum samples in pneumococcal pneumonia.口咽部肺炎球菌荚膜抗原的产生及肺炎球菌肺炎患者咳出痰液样本受污染的可能性。
Epidemiol Infect. 1993 Jun;110(3):621-31. doi: 10.1017/s0950268800051049.
4
Pneumococci in nasopharyngeal samples from Filipino children with acute respiratory infections.来自患有急性呼吸道感染的菲律宾儿童鼻咽样本中的肺炎球菌。
J Clin Microbiol. 1994 Dec;32(12):2948-52. doi: 10.1128/jcm.32.12.2948-2952.1994.
5
Detection of Bacteroides fragilis endotoxin in amniotic fluid by counterimmunoelectrophoresis.用对流免疫电泳法检测羊水脆弱拟杆菌内毒素
Antonie Van Leeuwenhoek. 1994;66(4):333-6. doi: 10.1007/BF00882769.
6
Detection of Pneumococcal Capsular polysaccharide antigens by latex agglutination, counterimmunoelectrophoresis, and radioimmunoassay in middle ear exudates in acute otitis media.通过乳胶凝集试验、对流免疫电泳和放射免疫测定法检测急性中耳炎中耳渗出液中的肺炎球菌荚膜多糖抗原。
J Clin Microbiol. 1980 Feb;11(2):135-40. doi: 10.1128/jcm.11.2.135-140.1980.
7
Diagnosis of pneumococcal pneumonia by coagglutination and counterimmunoelectrophoresis of sputum samples.通过痰液样本的协同凝集试验和对流免疫电泳诊断肺炎球菌肺炎。
Eur J Clin Microbiol. 1982 Apr;1(2):91-6. doi: 10.1007/BF02014198.
8
Minimum number of bacteria needed for antigen detection by counterimmunoelectrophoresis: in vivo and in vitro studies.对流免疫电泳检测抗原所需的最低细菌数量：体内和体外研究
J Clin Microbiol. 1981 Apr;13(4):681-7. doi: 10.1128/jcm.13.4.681-687.1981.
9
Effect of immunization with pneumolysin on survival time of mice challenged with Streptococcus pneumoniae.用肺炎溶血素免疫对肺炎链球菌攻击的小鼠存活时间的影响。
Infect Immun. 1983 May;40(2):548-52. doi: 10.1128/iai.40.2.548-552.1983.
10
Detection of Legionella pneumophila capsular-like envelope antigens by counterimmunoelectrophoresis.用对流免疫电泳法检测嗜肺军团菌荚膜样包膜抗原
J Clin Microbiol. 1981 Apr;13(4):637-42. doi: 10.1128/jcm.13.4.637-642.1981.
本文引用的文献
1
Bacteriologic flora of the lower respiratory tract.下呼吸道的细菌菌群
N Engl J Med. 1961 Dec 28;265:1273-8. doi: 10.1056/NEJM196112282652601.
2
Non-tuberculous bacterial flora of sputum and of the upper and lower respiratory tract in pulmonary tuberculosis.
Lancet. 1959 Dec 19;2(7112):1115-7. doi: 10.1016/s0140-6736(59)90100-x.
3
Observations on the carriage of pneumococci by patients with chronic bronchitis, and their families.慢性支气管炎患者及其家庭成员肺炎球菌携带情况的观察
J Hyg (Lond). 1955 Jun;53(2):225-33. doi: 10.1017/s0022172400000693.
4
POLYVALENT, DIAGNOSTIC PNEUMOCOCCUS SERA.多价诊断性肺炎球菌血清
Acta Pathol Microbiol Scand. 1963;59:533-6. doi: 10.1111/j.1699-0463.1963.tb01256.x.
5
Chronic bronchitis. Changes in the bacterial flora of the sputum associated with exacerbations and long-term antibacterial treatment.慢性支气管炎。痰液细菌菌群变化与病情加重及长期抗菌治疗的关系。
Br J Dis Chest. 1961 Jan;55:23-9. doi: 10.1016/s0007-0971(61)80020-x.
6
An evaluation of sputum examination in chronic bronchitis.慢性支气管炎痰液检查的评估
Lancet. 1957 Dec 28;273(7009):1306-9. doi: 10.1016/s0140-6736(57)91637-9.
7
Transtracheal aspiration in the evaluation of patients with pneumonia.经气管吸引术在肺炎患者评估中的应用
Ann Intern Med. 1970 Feb;72(2):183-7. doi: 10.7326/0003-4819-72-2-183.
8
Nosocomial respiratory infections with gram-negative bacilli. The significance of colonization of the respiratory tract.革兰氏阴性杆菌引起的医院获得性呼吸道感染。呼吸道定植的意义。
Ann Intern Med. 1972 Nov;77(5):701-6. doi: 10.7326/0003-4819-77-5-701.
9
10
The John Barnwell Lecture. The chemotherapy of bacterial respiratory infections.约翰·巴恩韦尔讲座。细菌性呼吸道感染的化疗
Am Rev Respir Dis. 1970 Jun;101(6):841-59. doi: 10.1164/arrd.1970.101.6.841.
Zotero 插件