Spencer R C, Savage M A
J Clin Pathol. 1976 Mar;29(3):187-90. doi: 10.1136/jcp.29.3.187.
The use of Counter immunoelectrophoresis (CIE) for the detection of pneumococcal capsular antigen in the sputum and serum of patients suffering from acute respiratory infections is described. The CIE of sputum gave positive results in 224 (99%) out of 225 samples in which Streptococcus pneumoniae was isolated by cultural techniques, and in 23 (9%) out of 262 samples in which no or other potential pathogens had been isolated. In the detection of capsular antigen in serum, CIE was positive in 32 (35%) out of 92 pneumonia cases and was associated with an increase in mortality.
本文描述了采用对流免疫电泳(CIE)检测急性呼吸道感染患者痰液和血清中肺炎球菌荚膜抗原的方法。在通过培养技术分离出肺炎链球菌的225份痰液样本中,224份(99%)的CIE检测结果呈阳性;在未分离出病原体或分离出其他潜在病原体的262份样本中,23份(9%)的CIE检测结果呈阳性。在血清荚膜抗原检测中,92例肺炎患者中有32例(35%)的CIE检测结果呈阳性,且与死亡率增加相关。