University of Geneva and University Hospitals of Geneva, 4 Rue Gabrielle-Perret-Gentil, 1211, Geneva 14, Switzerland.
University Hospitals of Geneva and Centre for Biomedical Imaging (CIBM), 4 Rue Gabrielle-Perret-Gentil, 1211, Geneva 14, Switzerland.
Int J Comput Assist Radiol Surg. 2016 May;11(5):733-43. doi: 10.1007/s11548-015-1260-8. Epub 2015 Jul 26.
The purpose of the present study is to apply kinetic analysis to investigate exercise-related changes in the metabolism of the skeletal muscle of the rat hindlimb by [[Formula: see text]]acetate positron emission tomography and computed tomography (PET/CT).
Contractions were induced in Wistar rats' left hindlimb by electrostimulation of the Vastus Lateralis muscle motor point. After 15 min of muscle contractions, [[Formula: see text]]acetate was injected and PET/CT of both hindlimbs was acquired. The resting hindlimb was used as a control reference. The kinetic parameters [Formula: see text] and [Formula: see text] were calculated for the target muscles (exercised and control) and correlated with the corresponding standardized uptake values (SUVs). The ratio between each kinetic parameter values and the SUV extracted for the exercised muscle and the muscle at rest was computed ([Formula: see text] and [Formula: see text], respectively).
Kinetic analysis quantitatively confirmed that net tracer uptake ([Formula: see text]) and washout ([Formula: see text]) were significantly higher in exercised muscles ([Formula: see text] for exercised muscles vs. [Formula: see text] for resting muscles, [Formula: see text]; [Formula: see text] for exercised muscle vs. [Formula: see text] for resting muscle, [Formula: see text]). On the other hand, SUV was not significantly different between active and inactive muscles ([Formula: see text] for exercised muscles vs. [Formula: see text] for resting muscles). Linear regression analysis revealed a good correlation ([Formula: see text]) between net tracer uptake ratio ([Formula: see text]) and the SUV ratio [Formula: see text]). A lower correlation was found between the net tracer washout ratio ([Formula: see text]) and the SUV ratio ([Formula: see text]).
The present study showed that kinetic modelling can detect changes between active and inactive skeletal muscles with a higher sensitivity with respect to the SUV, when performed with [[Formula: see text]]acetate PET/CT.
本研究旨在应用动力学分析，通过[[公式：见正文]]乙酰酸盐正电子发射断层扫描和计算机断层扫描（PET/CT）研究运动引起的大鼠后肢骨骼肌代谢变化。
通过电刺激大鼠股外侧肌运动点，诱导左后肢肌肉收缩。在肌肉收缩 15 分钟后，注射[[公式：见正文]]乙酰酸盐，并对双侧后肢进行 PET/CT 扫描。静息后肢作为对照参考。计算目标肌肉（运动和对照）的动力学参数[[公式：见正文]]和[[公式：见正文]]，并将其与相应的标准化摄取值（SUV）相关联。计算每个动力学参数值与提取的运动肌肉和休息肌肉的 SUV 之间的比值（分别为[[公式：见正文]]和[[公式：见正文]]）。
动力学分析定量证实，净示踪剂摄取量[[公式：见正文]]和洗脱量[[公式：见正文]]在运动肌肉中显著升高（运动肌肉的[[公式：见正文]]比休息肌肉的[[公式：见正文]]，[Formula: see text]；运动肌肉的[[公式：见正文]]比休息肌肉的[[公式：见文本]]，[Formula: see text]）。另一方面，SUV 在活跃和不活跃肌肉之间没有显著差异（运动肌肉的[[公式：见正文]]比休息肌肉的[[公式：见正文]]）。线性回归分析显示，净示踪剂摄取量比与 SUV 比之间存在良好的相关性（[Formula: see text]）。发现净示踪剂洗脱比与 SUV 比之间的相关性较低（[Formula: see text]）。
本研究表明，与 SUV 相比，[[公式：见正文]]乙酰酸盐 PET/CT 进行的动力学建模可以更敏感地检测活跃和不活跃骨骼肌之间的变化。
