Geng Tuoyu, Guan Xiaojing, Smith Edward J
Institute of Epigenetics and Epigenomics, Yangzhou University, Yangzhou, Jiangsu 225009, China College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu 225009, China Department of Animal and Poultry Sciences, Virginia Tech, Blacksburg, VA 24061, United States of America
Department of Animal and Poultry Sciences, Virginia Tech, Blacksburg, VA 24061, United States of America.
Poult Sci. 2015 Sep;94(9):2099-107. doi: 10.3382/ps/pev224. Epub 2015 Jul 27.
Antibody response, an important trait in both agriculture and biomedicine, plays a part in protecting animals from infection. Dissecting molecular basis of antibody response may improve artificial selection for natural disease resistance in livestock and poultry. A number of genetic markers associated with antibody response have been identified in the chicken and mouse by linkage-based association studies, which only define genomic regions by genetic markers but do not pinpoint genes for antibody response. In contrast, global expression profiling has been applied to define the molecular bases of a variety of biological traits through identification of differentially expressed genes (DEGs). Here, we employed Affimetrix GeneChip Chicken Genome Arrays to identify differentially expressed genes for antibody response to sheep red blood cells (SRBC) using chickens challenged with and without SRBC or chickens with high and low anti-SRBC titers. The DEGs include those with known (i.e., MHC class I and IgH genes) or unknown function in antibody response. Classification test of these genes suggested that the response of the chicken to intravenous injection of SRBC involved multiple biological processes, including response to stress or other different stimuli, sugar, carbohydrate or protein binding, and cell or soluble fraction, in addition to antibody response. This preliminary study thus provides an insight into molecular basis of antibody response to SRBC in the chicken.
抗体反应是农业和生物医学中的一个重要特征，在保护动物免受感染方面发挥着作用。剖析抗体反应的分子基础可能会改善家畜和家禽对自然疾病抗性的人工选择。通过基于连锁的关联研究，在鸡和小鼠中已经鉴定出一些与抗体反应相关的遗传标记，这些研究仅通过遗传标记定义基因组区域，而没有确定抗体反应的基因。相比之下，全局表达谱分析已被用于通过鉴定差异表达基因（DEG）来定义各种生物学特征的分子基础。在这里，我们使用Affimetrix GeneChip鸡基因组阵列，通过用或不用绵羊红细胞（SRBC）攻击的鸡或抗SRBC滴度高和低的鸡，来鉴定对SRBC抗体反应的差异表达基因。这些DEG包括在抗体反应中具有已知（即MHC I类和IgH基因）或未知功能的基因。对这些基因的分类测试表明，鸡对静脉注射SRBC的反应涉及多个生物学过程，除了抗体反应外，还包括对应激或其他不同刺激的反应、糖、碳水化合物或蛋白质结合以及细胞或可溶性部分。因此，这项初步研究为鸡对SRBC抗体反应的分子基础提供了见解。
