Department of Animal Science, Garmsar Branch, Islamic Azad University, Garmsar, Iran.
Sci Rep. 2022 Oct 12;12(1):17060. doi: 10.1038/s41598-022-20990-2.
The study was conducted to consider the anticoccidial and immunogenic effectivities of encapsulated organic acids and anticoccidial drugs in broilers reared on a reused litter infected with Eimeria spp. for simulating in-field exposure to avian coccidiosis. 525 mixed-sex one-day-old broiler chicks (Ross 308) were used in a 2 × 3 factorial experiment as a completely randomized design with seven experimental groups and five replicates of 15 chicks. The seven experimental groups were included: negative (uninfected; T) and positive (infected; T) control groups fed a diet without additive, and other infected groups (T-T) fed diets supplemented with 0.05% maduramicin, 0.02% diclazuril, 0.1% EOAs, 0.05% maduramicin and 0.1% EOAs, 0.02% diclazuril and 0.1% EOAs. During the experimental period, the evaluated parameters were European production efficiency factor (EPEF; at 22 days of age (d)), oocyst output per gram feces (OPG; at different ages), oocyst reduction rate (ORR; at 22-d), survival rate (SR; at 22-d), caecal lesion score (CLS at 22-d), sporulation percentage (SP; by in vitro anticoccidial tests), bloody diarrhea (BD; by scoring the bloody feces each morning from 13 to 31-d), immunity (humoral test at 28 and 35-d and cell-mediated test at 22-d), goblet cells analysis of the jejunum (GC; at 22-d) and anti-coccidiosis index (ACI; at 22-d). EOAs and anticoccidials, especially their simultaneous feeding improved (P < 0.05) broiler's EPEF, SR, OPG, ORR, SP, CLS, immunity and BD (scored). ACI was improved (P < 0.05) by EOAs more than anticoccidials (marked vs. moderate). The highest ACI was significantly observed in EOAs + diclazuril group. EOAs as a safe alternative had more intensive anticoccidial and immunogenic properties and increased the anticoccidial drugs' effectiveness, especially diclazuril in Eimeria spp-infected broilers.
这项研究旨在考虑在重复使用的受艾美耳球虫感染的垫料中饲养的肉鸡中，包被有机酸和抗球虫药物的抗球虫和免疫效力，以模拟田间暴露于禽球虫病。使用 525 只混合性别 1 日龄肉鸡（罗斯 308）进行 2×3 因子实验，完全随机设计，设七个实验组，每组五个重复，每个重复 15 只鸡。七个实验组包括：阴性（未感染；T）和阳性（感染；T）对照组饲喂无添加剂日粮，其他感染组（T-T）饲喂添加 0.05%马杜霉素、0.02%地克珠利、0.1%EOAs、0.05%马杜霉素和 0.1%EOAs、0.02%地克珠利和 0.1%EOAs 的日粮。在实验期间，评估的参数为欧洲生产效率因子（EPEF；在 22 日龄（d））、每克粪便排出的卵囊数（OPG；在不同年龄）、卵囊减少率（ORR；在 22-d）、存活率（SR；在 22-d）、盲肠病变评分（CLS 在 22-d）、孢子形成百分比（SP；通过体外抗球虫试验）、血性腹泻（BD；通过每天早上从 13 到 31-d 评分血性粪便）、免疫力（28 和 35-d 的体液试验和 22-d 的细胞介导试验）、空肠杯状细胞分析（GC；在 22-d）和抗球虫指数（ACI；在 22-d）。EOAs 和抗球虫药物，特别是它们的同时喂养，提高了（P<0.05）肉鸡的 EPEF、SR、OPG、ORR、SP、CLS、免疫力和 BD（评分）。ACI 提高（P<0.05）EOAs 多于抗球虫药物（标记与中度）。在 EOAs+地克珠利组观察到最高的 ACI。EOAs 作为一种安全的替代品，具有更强的抗球虫和免疫原性，增加了抗球虫药物的效果，特别是在感染艾美耳球虫的肉鸡中地克珠利。
