Department of Chemistry, Razi University, Kermanshah, Iran; Department of Chemistry, Bonab Research Center, BNRC, NSTRI, P.O. Box 56515-196, Bonab, East Azarbayjan, Iran.
Department of Chemistry, Razi University, Kermanshah, Iran.
Biosens Bioelectron. 2015 Dec 15;74:764-9. doi: 10.1016/j.bios.2015.07.032. Epub 2015 Jul 17.
Herein, a label free electrochemical aptasensor based on ordered mesoporous carbon-gold nanocomposite modified screen printed electrode has been fabricated for the detection of vascular endothelial growth factor (VEGF165) as a tumor marker. The electrochemical behavior of prepared biosensor was investigated by cyclic voltammetry and electrochemical impedance spectroscopy. The principle of operation of the proposed aptasensor is based on the changes in the interfacial properties of the electrode due to interaction of the immobilized antiVEGF165 aptamer at the electrode surface with VEGF165 tumor marker in the sample solution, which results in a change in the interfacial charge transfer resistance as detected by electrochemical impedance spectroscopy. The calibration curve for VEGF165 determination was linear over 10.0-300.0 pg mL(-1) with a limit of detection (3σ/S) of 1.0 pg mL(-1). The prepared aptasensor exhibited high sensitivity and good selectivity and reproducibility. The aptasensor was successfully applied to the determination of VEGF165 in serum sample of a lung cancer patient.
本文制备了一种基于有序介孔碳-金纳米复合材料修饰丝网印刷电极的无标记电化学适体传感器，用于检测血管内皮生长因子（VEGF165）作为肿瘤标志物。通过循环伏安法和电化学阻抗谱研究了制备的生物传感器的电化学行为。该拟议适体传感器的工作原理基于固定在电极表面的抗 VEGF165 适体与样品溶液中 VEGF165 肿瘤标志物相互作用导致电极界面性质发生变化，这导致通过电化学阻抗谱检测到界面电荷转移电阻发生变化。VEGF165 测定的校准曲线在 10.0-300.0 pg mL(-1) 范围内呈线性，检测限（3σ/S）为 1.0 pg mL(-1)。所制备的适体传感器表现出高灵敏度、良好的选择性和重现性。该适体传感器成功应用于肺癌患者血清样品中 VEGF165 的测定。
