Lhomme Sebastien, Abravanel Florence, Dubois Martine, Chapuy-Regaud Sabine, Sandres-Saune Karine, Mansuy Jean-Michel, Rostaing Lionel, Kamar Nassim, Izopet Jacques
INSERM, UMR1043, Toulouse F-31300, France; Department of Virology, National Reference Center for Hepatitis E Virus, CHU Purpan, Toulouse F-31300, France; Université Paul Sabatier, Toulouse, France.
Department of Virology, National Reference Center for Hepatitis E Virus, CHU Purpan, Toulouse F-31300, France.
Infect Genet Evol. 2015 Oct;35:50-5. doi: 10.1016/j.meegid.2015.07.028. Epub 2015 Jul 26.
Southwest France is a highly endemic region for hepatitis E virus (HEV). This study examined the circulation of HEV strains between 2003 and 2014 in the Midi-Pyrénées, and compared these data with those from the rest of France. The polyproline region (PPR) of the ORF1 region of the HEV genome was also analyzed. HEV genotype was determined by sequencing a 348-nt fragment within the ORF2 gene for 333 strains in the Midi-Pyrénées and for 571 strains from the rest of France. PPR region was characterized for 56 strains. The frequency of subgenotype 3f decreased over time, whereas subgenotype 3c increased in the Midi-Pyrénées. Repartition of strains did not differ in the Midi-Pyrénées compared to the rest of France. HEV3i and HEV4 have been recently detected throughout France. PPR lengths showed that two major groups of HEV3f exist. Our study shows that HEV3 distribution in the Midi-Pyrénées was similar to the whole of France. Local dietary habits could explain the higher seroprevalence in the Midi-Pyrénées rather the circulation of a particular variant in this region.
法国西南部是戊型肝炎病毒（HEV）的高流行地区。本研究调查了2003年至2014年期间中比利牛斯地区HEV毒株的传播情况，并将这些数据与法国其他地区的数据进行了比较。还分析了HEV基因组ORF1区域的多脯氨酸区域（PPR）。通过对中比利牛斯地区的333株毒株以及法国其他地区的571株毒株的ORF2基因内348个核苷酸的片段进行测序来确定HEV基因型。对56株毒株的PPR区域进行了特征分析。3f亚型的频率随时间下降，而3c亚型在中比利牛斯地区增加。与法国其他地区相比，中比利牛斯地区毒株的分布没有差异。最近在法国各地都检测到了HEV3i和HEV4。PPR长度表明存在两组主要的HEV3f。我们的研究表明，中比利牛斯地区的HEV3分布与法国整体相似。当地的饮食习惯可能解释了中比利牛斯地区较高的血清阳性率，而不是该地区特定变异株的传播。
