Zhihong N, Yun F, Pinggui Z, Sulian Z, Zhang Aijun
Department of Obstetrics and Gynecology, IVF Unit, Ruijin Hospital Affiliated to Shanghai Jiaotong University, Shanghai, China.
Department of Obstetrics and Gynecology, IVF Unit, Ruijin Hospital Affiliated to Shanghai Jiaotong University, Shanghai, China
Reprod Sci. 2016 Jan;23(1):124-33. doi: 10.1177/1933719115597761. Epub 2015 Aug 2.
In this study, we aimed to clarify the inflammatory cytokine profile of endometrium in patients with adenomyosis during the implantation window after ovarian stimulation. Eighteen patients with adenomyosis and 24 control patients undergoing in vitro fertilization treatment were included in this prospective case-control study. Regular gonadotropin-releasing hormone antagonist protocol was used for ovarian stimulation. Endometrial samples were obtained 7 days after human chorionic gonadotropin (hCG) injection (hCG + 7). Cytokine levels in endometrium secretions from women with and without adenomyosis were assayed by multiplex immunoassay, levels of interleukin (IL) 6 (25.9 ± 6.6 vs 12.4 ± 3.4 pg/mL; P = .001), IL-10 (10.4 ± 2.9 vs 15.6 ± 4.2 pg/mL; P = .001), IL-17 (11.9 ± 3.0 vs 14.2 ± 3.9 pg/mL; P = .046), interferon-γ (11.7 ± 3.5 vs 8.0 ± 3.4 pg/mL; P = .001), and monocyte chemoattractant protein-1 (MCP-1; 37.1 ± 6.5 vs 16.4 ± 3.2 pg/mL; P = .001) were significantly different between patients with adenomyosis and control groups, respectively. Immunohistochemistry and quantitative real-time polymerase chain reaction showed that CD-68+, IL-6, and MCP-1 expression were higher and IL-10 was lower in adenomyosis endometrium epithelia compared to controls. In conclusion, within the implantation window of ovarian stimulation cycles, macrophages, IL-6, IL-10, and MCP-1 are expressed differently in the endometrium of women with adenomyosis, which may correlate with compromised endometrium receptivity. We postulated that cytokines of endometrial secretions expressed differently in patients with adenomyosis may contribute to impaired endometrium receptivity in these patients.
在本研究中，我们旨在阐明子宫腺肌病患者在卵巢刺激后的着床窗期子宫内膜的炎性细胞因子谱。本前瞻性病例对照研究纳入了18例子宫腺肌病患者和24例接受体外受精治疗的对照患者。采用常规促性腺激素释放激素拮抗剂方案进行卵巢刺激。在注射人绒毛膜促性腺激素（hCG）7天后（hCG + 7）获取子宫内膜样本。通过多重免疫测定法检测有和没有子宫腺肌病女性子宫内膜分泌物中的细胞因子水平，子宫腺肌病患者组和对照组之间，白细胞介素（IL）-6（25.9±6.6 vs 12.4±3.4 pg/mL；P = .001）、IL-10（10.4±2.9 vs 15.6±4.2 pg/mL；P = .001）、IL-17（11.9±3.0 vs 14.2±3.9 pg/mL；P = .046）、干扰素-γ（11.7±3.5 vs 8.0±3.4 pg/mL；P = .001）和单核细胞趋化蛋白-1（MCP-1；37.1±6.5 vs 16.4±3.2 pg/mL；P = .001）水平存在显著差异。免疫组织化学和定量实时聚合酶链反应显示，与对照组相比，子宫腺肌病患者子宫内膜上皮中CD-68+、IL-６和MCP-1的表达较高，而IL-10的表达较低。总之，在卵巢刺激周期的着床窗期内，巨噬细胞、IL-6、IL-10和MCP-1在子宫腺肌病女性的子宫内膜中表达不同，这可能与子宫内膜容受性受损有关。我们推测，子宫腺肌病患者子宫内膜分泌物中表达不同的细胞因子可能导致这些患者子宫内膜容受性受损。
