Buathong Saiwasan, Leelayoova Saovanee, Mungthin Mathirut, Naaglor Tawee, Taamasri Paanjit, Suwannahitatorn Picha, Tan-Ariya Peerapan
Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand.
Parasitol Res. 2015 Sep;114(9):3547-9. doi: 10.1007/s00436-015-4640-7. Epub 2015 Aug 5.
Opisthorchis viverrini is highly prevalent throughout Southeast Asia. Chronic infection of this parasite leads to cholangiocarcinoma (CCA), a fatal bile duct cancer. The early and accurate detection of this parasite is very important; therefore, new PCR methods targeting cytochrome c oxidase subunit one and NADH dehydrogenase subunit one gene to detect O. viverrini in fecal specimens have been developed. Ninety O. viverrini-positive human fecal samples were used in this study. The PCR sensitivity of both genes was compared with internal transcribe spacer 2 (ITS2)-PCR. The sensitivity of cox1-PCR and nad1-PCR was 66.7 and 50 %, respectively. The sensitivity of cox1-PCR and nad1-PCR achieved 89.1 and 71.7 % in specimens containing O. viverrini eggs of >100 eggs per gram (EPG). Additionally, these primers can be used to provide the information on genetic diversity from mitochondrial genes of O. viverrini.
猫后睾吸虫在东南亚地区高度流行。这种寄生虫的慢性感染会导致胆管癌（CCA），一种致命的胆管癌。早期准确检测这种寄生虫非常重要；因此，已经开发出针对细胞色素c氧化酶亚基1和NADH脱氢酶亚基1基因的新PCR方法，用于检测粪便样本中的猫后睾吸虫。本研究使用了90份猫后睾吸虫阳性的人类粪便样本。将这两个基因的PCR敏感性与内转录间隔区2（ITS2）-PCR进行比较。cox1-PCR和nad1-PCR的敏感性分别为66.7%和50%。在每克含有>100个虫卵（EPG）的猫后睾吸虫虫卵的样本中，cox1-PCR和nad1-PCR的敏感性分别达到89.1%和71.7%。此外，这些引物可用于提供猫后睾吸虫线粒体基因的遗传多样性信息。
