Ajona D, Pajares M J, Chiara M D, Rodrigo J P, Jantus-Lewintre E, Camps C, Suarez C, Bagán J V, Montuenga L M, Pio R
Program in Solid Tumors and Biomarkers, Center for Applied Medical Research (CIMA), Pamplona, Spain.
Navarra's Health Research Institute (IDISNA), Pamplona, Spain.
Oral Dis. 2015 Oct;21(7):899-904. doi: 10.1111/odi.12363. Epub 2015 Sep 7.
Complement C4d-containing fragments have been proposed as diagnostic markers for lung cancer. The purpose of this study was to evaluate the presence of C4d in oropharyngeal (OPSCC) and oral (OSCC) squamous cell carcinomas.
C4d staining was analyzed by immunohistochemistry in 244 OPSCC surgical specimens. C4d levels were quantified by ELISA in resting saliva samples from 48 patients with oral leukoplakia and 62 with OSCC. Plasma samples from 21 patients with leukoplakia and 30 with oral carcinoma were also studied.
C4d staining in OPSCC specimens was associated with nodal invasion (P = 0.001), histopathologic grade (P = 0.014), disease stage (P = 0.040), and focal-adhesion kinase expression (P < 0.001). No association was found between C4d and prognosis. Saliva C4d levels were higher in patients with oral cancer than in subjects with leukoplakia (0.07 ± 0.07 vs 0.04 ± 0.03 μg ml(-1) , P = 0.003). The area under the ROC curve was 0.63 (95%CI: 0.55-0.71). Salivary C4d levels in stage IV patients were higher than in patients with earlier stages (P = 0.028) and correlated with tumor size (P = 0.045). Plasma C4d levels also correlated with salivary C4d levels (P = 0.041), but differences between patients with oral cancer and subjects with leukoplakia were not significant (1.26 ± 0.59 vs 1.09 ± 0.39 μg ml(-1) , P = 0.232).
C4d-containing fragments are detected in oral primary tumors and are increased in saliva from patients with OSCC.
含补体C4d的片段已被提议作为肺癌的诊断标志物。本研究的目的是评估口咽鳞状细胞癌（OPSCC）和口腔鳞状细胞癌（OSCC）中C4d的存在情况。
采用免疫组织化学方法分析244例OPSCC手术标本中的C4d染色情况。通过酶联免疫吸附测定（ELISA）对48例口腔白斑患者和62例OSCC患者的静息唾液样本中的C4d水平进行定量分析。还研究了21例白斑患者和30例口腔癌患者的血浆样本。
OPSCC标本中的C4d染色与淋巴结转移（P = 0.001）、组织病理学分级（P = 0.014）、疾病分期（P = 0.040）以及粘着斑激酶表达（P < 0.001）相关。未发现C4d与预后之间存在关联。口腔癌患者唾液中的C4d水平高于白斑患者（0.07±0.07 vs 0.04±0.03μg/ml，P = 0.003）。ROC曲线下面积为0.63（95%CI：0.55 - 0.71）。IV期患者的唾液C4d水平高于早期患者（P = 0.028），且与肿瘤大小相关（P = 0.045）。血浆C4d水平也与唾液C4d水平相关（P = 0.041），但口腔癌患者与白斑患者之间的差异不显著（1.26±0.59 vs 1.09±0.
