Kazemian Hossein, Ghafourian Sobhan, Heidari Hamid, Amiri Pouya, Yamchi Jalil Kardan, Shavalipour Aref, Houri Hamidreza, Maleki Abbas, Sadeghifard Nourkhoda
Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, IR.
Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IR.
Rev Soc Bras Med Trop. 2015 Jul-Aug;48(4):432-6. doi: 10.1590/0037-8682-0065-2015.
Chamomile (Chamaemelum nobile) is widely used throughout the world, and has anti-inflammatory, deodorant, bacteriostatic, antimicrobial, carminative, sedative, antiseptic, anti-catarrhal, and spasmolytic properties. Because of the increasing incidence of drug-resistant bacteria, the development of natural antibacterial sources such as medical herbs for the treatment of infectious diseases is necessary. Extracts from different plant parts such as the leaves, flowers, fruit, and bark of Combretum albiflorum, Laurus nobilis , and Sonchus oleraceus were found to possess anti-quorum sensing (QS) activities. In this study, we evaluated the effect of C. nobile against Pseudomonas aeruginosa biofilm formation.
The P. aeruginosa samples were isolated from patients with different types of infection, including wound infection, septicemia, and urinary tract infection. The flowers of C. nobile were dried and the extract was removed using a rotary device and then dissolved in dimethyl sulfoxide at pH 7.4. The microdilution method was used to evaluate the minimum inhibitory concentration (MIC) of this extract on P. aeruginosa , and biofilm inhibition was assayed.
Eighty percent of the isolated samples (16/20) could form a biofilm, and most of these were isolated from wound infections. The biofilm inhibitory concentration of the C. nobile extract was 6.25-25mg/ml, whereas the MIC was 12.5-50mg/ml.
The anti-QS property of C. nobile may play an important role in its antibacterial activity, thus offering an additional strategy in the fight against bacterial infections. However, molecular investigation is required to explore the exact mechanisms of the antibacterial action and functions of this phytocompound.
洋甘菊（果香菊）在全球被广泛使用，具有抗炎、除臭、抑菌、抗菌、驱风、镇静、防腐、抗卡他和解痉特性。由于耐药菌的发病率不断上升，开发天然抗菌源如草药用于治疗传染病是必要的。已发现白花风车子、月桂和苦苣菜等不同植物部位（如叶子、花朵、果实和树皮）的提取物具有抗群体感应（QS）活性。在本研究中，我们评估了洋甘菊对铜绿假单胞菌生物膜形成的影响。
铜绿假单胞菌样本从不同类型感染的患者中分离，包括伤口感染、败血症和尿路感染。将洋甘菊的花朵干燥，使用旋转装置提取提取物，然后将其溶解在pH 7.4的二甲基亚砜中。采用微量稀释法评估该提取物对铜绿假单胞菌的最低抑菌浓度（MIC），并检测生物膜抑制情况。
80%的分离样本（16/20）能够形成生物膜，其中大多数分离自伤口感染。洋甘菊提取物的生物膜抑制浓度为6.25 - 25mg/ml，而MIC为12.5 - 50mg/ml。
洋甘菊的抗群体感应特性可能在其抗菌活性中起重要作用，从而为对抗细菌感染提供了一种额外策略。然而，需要进行分子研究以探索这种植物化合物抗菌作用和功能的确切机制。
