Ellango R, Singh Shalini Thakur, Rana Vipin Singh, Gayatri Priya N, Raina Harpreet, Chaubey Rahul, Naveen N C, Mahmood Riaz, Ramamurthy V V, Asokan R, Rajagopal R
Division of Biotechnology, Indian Institute of Horticultural Research, Bangalore, India-560089. Contributed Equally.
Contributed Equally. Department of Zoology, University of Delhi, Delhi, India-110007.
Environ Entomol. 2015 Aug;44(4):1258-64. doi: 10.1093/ee/nvv062. Epub 2015 May 11.
The whitefly Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) is a phloem-feeding, economically important pest of crops worldwide. In addition to direct damage, it also vectors a number of plant viruses belonging to the family Geminiviridae. Its populations differ biologically with respect to insecticide resistance, virus transmission and host range. Therefore, understanding genetic variation among populations is important for management. We sequenced 850 bp of the mitochondrial COI (mtCOI) gene from B. tabaci populations surveyed across India. BLAST analysis of the mtCOI sequences generated in this study with sequences from the mtCOI dataset showed the presence of one invasive group, MEAM1, and eight other groups of B. tabaci in India. mtCOI sequence analyses showed the presence of Asia I, Asia I-India, Asia II-1, Asia II-5, Asia II-7, Asia II-8, and Asia II-11 genetic groups. We also found China-3 in a field in Birbhum district, West Bengal, India, suggesting a role of anthropogenic activities in the distribution of B. tabaci. Interestingly, more than one genetic group was found coexisting in the same field.
烟粉虱Bemisia tabaci（Gennadius）（半翅目：粉虱科）是一种以韧皮部为食的害虫，对全球农作物具有重要经济影响。除了造成直接损害外，它还传播多种属于双生病毒科的植物病毒。其种群在抗药性、病毒传播和寄主范围方面存在生物学差异。因此，了解种群间的遗传变异对于害虫管理至关重要。我们对印度各地调查的烟粉虱种群的线粒体细胞色素氧化酶亚基I（mtCOI）基因的850bp进行了测序。本研究生成的mtCOI序列与mtCOI数据集的序列进行BLAST分析，结果显示印度存在一个入侵种群MEAM1和其他八个烟粉虱种群。mtCOI序列分析显示存在亚洲I、亚洲I-印度、亚洲II-1、亚洲II-5、亚洲II-7、亚洲II-8和亚洲II-11遗传组。我们还在印度西孟加拉邦比尔布姆区的一块田地中发现了中国-3，这表明人为活动在烟粉虱分布中起到了作用。有趣的是，在同一田地中发现了不止一个遗传组共存。
