Longwe Herbert, Phiri Kamija S, Mbeye Nyanyiwe M, Gondwe Thandile, Jambo Kondwani C, Mandala Wilson L
Department of Basic Medical Sciences, College of Medicine, University of Malawi, Blantyre, Malawi.
Tropical Haematology Research Unit, College of Medicine, University of Malawi, Blantyre, Malawi.
BMC Immunol. 2015 Aug 28;16:50. doi: 10.1186/s12865-015-0115-y.
As a result of successful PMTCT programs, children born from HIV-infected mothers are now effectively protected from contracting the infection. However, it is not well known whether in utero exposure to the virus and the subsequent exposure to Cotrimoxazole (CTX) prophylaxis affect the cell mediated immune system of the children. This observational prospective study was aimed at determining how CD4(+) T, CD8(+) T and B cell subsets varied in HIV-exposed but uninfected (HEU) children at different ages.
We recruited HEU and HIV-unexposed and uninfected (HUU) children from 6 months of age and followed them up until they were 18 months old. HEU children received daily CTX prophylaxis beginning at 6 weeks of age until when 12 months of age. Venous blood samples were collected 6 monthly and analysed for different subsets of CD8(+) T, B cells and totalCD4(+) T cells.
At 6 months of age, HEU children had a lower percentage of total CD4(+) T cells compared to HUU children and a lower proportion of naïve CD8(+) T cells but higher percentage of effector memory CD8(+) T cells compared to HUU children. HEU and HUU children had similar proportions of all B cell subsets at all ages.
The study showed that the subtle variations in CD4(+) and CD8(+) T cell subsets observed at 6 months do not last beyond 12 months of age, suggesting that HEU children have a robust cell-mediated immune system during first year of life.
This article report is not based on results of a controlled health-care intervention.
由于成功实施了预防母婴传播（PMTCT）项目，感染艾滋病毒的母亲所生的儿童现在能有效避免感染该病毒。然而，子宫内接触病毒以及随后接触复方新诺明（CTX）预防措施是否会影响儿童的细胞介导免疫系统，目前尚不清楚。这项观察性前瞻性研究旨在确定不同年龄的艾滋病毒暴露但未感染（HEU）儿童的CD4(+) T细胞、CD8(+) T细胞和B细胞亚群如何变化。
我们招募了6个月大的HEU儿童以及未接触艾滋病毒且未感染（HUU）的儿童，并对他们进行随访直至18个月大。HEU儿童从6周龄开始每日接受CTX预防，直至12个月龄。每6个月采集一次静脉血样，分析CD8(+) T细胞、B细胞和总CD4(+) T细胞的不同亚群。
在6个月大时，与HUU儿童相比，HEU儿童的总CD4(+) T细胞百分比更低，幼稚CD8(+) T细胞比例更低，但效应记忆CD8(+) T细胞百分比更高。在所有年龄段，HEU儿童和HUU儿童的所有B细胞亚群比例相似。
该研究表明，6个月时观察到的CD4(+)和CD8(+) T细胞亚群的细微变化在12个月龄后并未持续，这表明HEU儿童在生命的第一年具有强大的细胞介导免疫系统。
本文报告并非基于对照医疗干预的结果。
