Zhu Qili, Zhou Benguo, Gao Zhengliang, Liang Yuancun
Department of Plant Pathology, Shandong Agricultural University, Taian, 271018, China.
Tobacco Research Institute, Anhui Academy of Agricultural Sciences, Hefei, 230031, China.
Curr Microbiol. 2015 Dec;71(6):632-7. doi: 10.1007/s00284-015-0901-z. Epub 2015 Aug 28.
Phospholipase C (PLC) plays important roles in regulating various biological processes in eukaryotes. Currently, little is known about the function of PLC in filamentous fungi, especially the plant pathogenic fungi. Fusarium graminearum is the causal agent of Fusarium head blight in many cereal crops. BLAST search revealed that Fusarium genome contains six FgPLC genes. Using quantitative RT-PCR, different FgPLC gene expressions in mycelia were analyzed. To investigate the role of FgPLC in F. graminearum biology, a pharmacological study using a known inhibitor of PLC (U73122) was conducted. Results showed that inhibition of FgPLC resulted in significant alterations of mycelial growth, conidiation, conidial germination, perithecium formation, and expressions of Tri5 and Tri6 genes. As expected, the treatment of F. graminearum with U73343, an inactive analog of U73122, showed no effect on F. graminearum biology. Our results suggested strongly that FgPLC plays important roles in F. graminearum growth and development.
磷脂酶C（PLC）在真核生物中调节各种生物学过程中发挥着重要作用。目前，关于PLC在丝状真菌，尤其是植物病原真菌中的功能知之甚少。禾谷镰刀菌是许多谷类作物中镰刀菌穗腐病的病原体。BLAST搜索显示，镰刀菌基因组包含六个FgPLC基因。使用定量RT-PCR分析了菌丝体中不同FgPLC基因的表达。为了研究FgPLC在禾谷镰刀菌生物学中的作用，使用已知的PLC抑制剂（U73122）进行了药理学研究。结果表明，抑制FgPLC会导致菌丝生长、产孢、孢子萌发、子囊壳形成以及Tri5和Tri6基因表达的显著改变。正如预期的那样，用U73122的无活性类似物U73343处理禾谷镰刀菌对其生物学特性没有影响。我们的结果强烈表明，FgPLC在禾谷镰刀菌的生长和发育中起着重要作用。
