Goldfarb A H, Garten R S, Waller J, Labban J D
Department of Kinesiology, University of North Carolina Greensboro, Greensboro, NC 27402-6170, USA.
Geriatric Research, Education, and Clinical Center, Salt Lake City VAMC, Salt Lake City, UT 84148, USA.
J Biomark. 2014;2014:248313. doi: 10.1155/2014/248313. Epub 2014 Oct 15.
The present study aimed to determine the day to day variability and reliability of several blood oxidative stress markers at rest in a healthy young cohort over a four-week period. Twelve apparently healthy resistance trained males (24.6 ± 3.0 yrs) were tested over 7 visits within 4 weeks with at least 72 hrs between visits at the same time of day. Subjects rested 30 minutes prior to blood being obtained by vacutainer. Results. The highest IntraClass correlations (ICC's) were obtained for protein carbonyls (PC) and oxygen radical absorbance capacity (ORAC) (PC = 0.785 and ORAC = 0.780). Cronbach's α reliability score for PC was 0.967 and for ORAC was 0.961. The ICC's for GSH, GSSG, and the GSSG/TGH ratio ICC were 0.600, 0.573, and 0.570, respectively, with Cronbach's α being 0.913, 0.904, and 0.903, respectively. Xanthine oxidase ICC was 0.163 and Cronbach's α was 0.538. Conclusions. PC and ORAC demonstrated good to excellent reliability while glutathione factors had poor to excellent reliability. Xanthine oxidase showed poor reliability and high variability. These results suggest that the PC and ORAC markers were the most stable and reliable oxidative stress markers in blood and that daily changes across visits should be considered when interpreting resting blood oxidative stress markers.
本研究旨在确定健康年轻人群在四周内静息状态下几种血液氧化应激标志物的每日变异性和可靠性。12名明显健康的进行抗阻训练的男性（24.6±3.0岁）在4周内接受了7次测试，每次测试在一天中的同一时间进行，且两次测试之间至少间隔72小时。受试者在通过真空采血管采集血液前休息30分钟。结果。蛋白质羰基（PC）和氧自由基吸收能力（ORAC）的组内相关系数（ICC）最高（PC = 0.785，ORAC = 0.780）。PC的克朗巴哈α信度得分为0.967，ORAC的为0.961。谷胱甘肽（GSH）、氧化型谷胱甘肽（GSSG）以及GSSG/TGH比值的ICC分别为0.600、0.573和0.570，相应的克朗巴哈α分别为0.913、0.904和0.903。黄嘌呤氧化酶的ICC为0.163，克朗巴哈α为0.538。结论。PC和ORAC显示出良好到优秀的可靠性，而谷胱甘肽相关因子的可靠性则从较差到优秀。黄嘌呤氧化酶显示出较差的可靠性和高变异性。这些结果表明，PC和ORAC标志物是血液中最稳定、可靠的氧化应激标志物，在解释静息血液氧化应激标志物时应考虑各次测试间的每日变化。
