College of Food Science and Technology, Nanjing Agricultural University , Nanjing 210095, People's Republic of China.
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences , Kunming, Yunnan 650201, People's Republic of China.
J Agric Food Chem. 2015 Sep 16;63(36):7986-94. doi: 10.1021/acs.jafc.5b03306. Epub 2015 Sep 4.
Sinonovacula constricta has been widely used as a health food and medicine in China, Japan, and Korea. In the present study, a water-soluble polysaccharide fraction (SCP-1) was prepared from S. constricta by enzyme-assisted extraction and purification of chromatography with DEAE-52 cellulose anion-exchange column and Sephadex G-100 size exclusion column. On the basis of the analytical results of high-performance liquid chromatography, Fourier transform-infrared spectroscopy, methylation analysis, and NMR spectroscopy, SCP-1 was found to have an average molecular weight of 15.63 kDa and a linear backbone of (1→4)-linked α-D-Glcp residue with one branch, α-D-Glcp, attached to the main chain by a (1→6) glycosidic bond at every five α-D-Glcp units. Furthermore, it was found that SCP-1 could significantly increase the viability of macrophages, enhance the capability of macrophage phagocytosis, increase the activity of acid phosphatase, and promote the production of nitric oxide, mouse tumor necrosis factor (TNF)-α, mouse interferon (IFN)-γ, and mouse interleukin (IL)-1β. The results suggest that SCP-1 possesses potent immunomodulating effect and may be explored as a potential biological response modifier.
在中国、日本和韩国，西施舌已被广泛用作保健食品和药品。本研究采用酶辅助提取法，并结合 DEAE-52 纤维素阴离子交换柱层析和 Sephadex G-100 凝胶排阻层析对西施舌进行分离纯化，得到一种水溶性多糖组分（SCP-1）。根据高效液相色谱、傅里叶变换红外光谱、甲基化分析和 NMR 光谱等分析结果，发现 SCP-1 的平均分子量为 15.63 kDa，其线性主链由（1→4）-连接的α-D-Glcp 残基组成，每隔五个α-D-Glcp 单元通过（1→6）糖苷键连接一个α-D-Glcp 支链。此外，SCP-1 能显著提高巨噬细胞活力，增强巨噬细胞吞噬能力，提高酸性磷酸酶活性，并促进一氧化氮、小鼠肿瘤坏死因子（TNF）-α、小鼠干扰素（IFN）-γ和小鼠白细胞介素（IL）-1β的产生。这些结果表明 SCP-1 具有潜在的免疫调节作用，可作为一种有希望的生物反应调节剂进行探索。
