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海生玫瑰弧菌新种,从海水中分离得到。

Roseivirga marina sp. nov., isolated from seawater.

作者信息

Pan Jie, Sun Cong, Wang Rui-Jun, Wu Min

机构信息

College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.

Ocean College, Zhejiang University, Hangzhou 310058, PR China.

出版信息

Int J Syst Evol Microbiol. 2015 Nov;65(11):4239-4243. doi: 10.1099/ijsem.0.000568. Epub 2015 Aug 27.

Abstract

Strain PSRT was isolated from seawater of the Pacific Ocean. Cells of the strain were Gram-stain-negative, strictly aerobic, rod-shaped and motile by gliding. Growth was observed at 4-40 °C (optimum 25-30 °C), at pH 6.0-9.5 (optimum pH 7.0-7.5) and with 0.5-8 % (w/v) NaCl (optimum 2-3 %). The major fatty acids were iso-C15 : 1 G (18.9 %), iso-C15 : 0 (26.3 %) and iso-C17 : 0 3-OH (17.9 %). The predominant isoprenoid quinone was MK-7, and the DNA G+C content was 49.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain PSRT was most closely related to Roseivirga spongicola UST030701-084T (96.9 % 16S rRNA gene sequence similarity), and they formed a distinct clade in neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees with significant bootstrap supports. Based on phenotypic, chemotaxonomic and phylogenetic characteristics, strain PSRT represents a novel species of the genus Roseivirga, for which the name Roseivirga marina sp. nov. is proposed. The type strain is PSRT ( = MCCC 1K00459T = KCTC 42444T).

摘要

菌株PSRT从太平洋海水中分离得到。该菌株细胞革兰氏染色阴性,严格好氧,杆状,通过滑动运动。在4-40 °C(最适温度25-30 °C)、pH值6.0-9.5(最适pH值7.0-7.5)和0.5-8 %(w/v)NaCl(最适2-3 %)条件下可生长。主要脂肪酸为异-C15 : 1 G(18.9 %)、异-C15 : 0(26.3 %)和异-C17 : 0 3-OH(17.9 %)。主要的类异戊二烯醌为MK-7,DNA的G+C含量为49.3 mol%。基于16S rRNA基因序列的系统发育分析表明,菌株PSRT与海绵玫瑰红菌UST030701-084T关系最为密切(16S rRNA基因序列相似性为96.9 %),在邻接法、最大似然法和最大简约法系统发育树中,它们形成了一个有显著自展支持的独特分支。基于表型、化学分类和系统发育特征,菌株PSRT代表玫瑰红菌属的一个新物种,为此提出新名称海洋玫瑰红菌(Roseivirga marina sp. nov.)。模式菌株为PSRT( = MCCC 1K00459T = KCTC 42444T)。

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