Pan Jie, Sun Cong, Wang Rui-Jun, Wu Min
College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
Ocean College, Zhejiang University, Hangzhou 310058, PR China.
Int J Syst Evol Microbiol. 2015 Nov;65(11):4239-4243. doi: 10.1099/ijsem.0.000568. Epub 2015 Aug 27.
Strain PSRT was isolated from seawater of the Pacific Ocean. Cells of the strain were Gram-stain-negative, strictly aerobic, rod-shaped and motile by gliding. Growth was observed at 4-40 °C (optimum 25-30 °C), at pH 6.0-9.5 (optimum pH 7.0-7.5) and with 0.5-8 % (w/v) NaCl (optimum 2-3 %). The major fatty acids were iso-C15 : 1 G (18.9 %), iso-C15 : 0 (26.3 %) and iso-C17 : 0 3-OH (17.9 %). The predominant isoprenoid quinone was MK-7, and the DNA G+C content was 49.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain PSRT was most closely related to Roseivirga spongicola UST030701-084T (96.9 % 16S rRNA gene sequence similarity), and they formed a distinct clade in neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees with significant bootstrap supports. Based on phenotypic, chemotaxonomic and phylogenetic characteristics, strain PSRT represents a novel species of the genus Roseivirga, for which the name Roseivirga marina sp. nov. is proposed. The type strain is PSRT ( = MCCC 1K00459T = KCTC 42444T).
菌株PSRT从太平洋海水中分离得到。该菌株细胞革兰氏染色阴性，严格好氧，杆状，通过滑动运动。在4-40 °C（最适温度25-30 °C）、pH值6.0-9.5（最适pH值7.0-7.5）和0.5-8 %（w/v）NaCl（最适2-3 %）条件下可生长。主要脂肪酸为异-C15 : 1 G（18.9 %）、异-C15 : 0（26.3 %）和异-C17 : 0 3-OH（17.9 %）。主要的类异戊二烯醌为MK-7，DNA的G+C含量为49.3 mol%。基于16S rRNA基因序列的系统发育分析表明，菌株PSRT与海绵玫瑰红菌UST030701-084T关系最为密切（16S rRNA基因序列相似性为96.9 %），在邻接法、最大似然法和最大简约法系统发育树中，它们形成了一个有显著自展支持的独特分支。基于表型、化学分类和系统发育特征，菌株PSRT代表玫瑰红菌属的一个新物种，为此提出新名称海洋玫瑰红菌（Roseivirga marina sp. nov.）。模式菌株为PSRT（ = MCCC 1K00459T = KCTC 42444T）。
