Chirmule N, Saxinger C, Pahwa S
Department of Pediatrics, North Shore University Hospital, Cornell University Medical College, Manhasset, NY 11030.
FEMS Microbiol Immunol. 1989 Apr;1(5):271-8. doi: 10.1111/j.1574-6968.1989.tb02392.x.
The human immunodeficiency virus (HIV-1) is known to be profoundly immunosuppressive [Spickett and Dalgleish (1988) Clin. Exp. Immunol. 71, 1]. In this communication, we have studied the influences of HIV-1 (BH10), HIV-2 (LAV-2) and STLV-3 on B and T cells from healthy volunteers. B lymphocytes were found to differentiate into immunoglobulin secreting cells in response to stimulation by proteins of HIV-1 and LAV-2, but not by STLV-3. This response was obtained at protein concentrations of 0.05-0.005 micrograms/ml and was T cell dependent. IgM secretion was induced only by HIV-1 in the EBV-transformed B cell line SKW 6.4. At higher concentrations all three retroviral preparations had inhibitory influences on functions of B as well as T lymphocytes. B cell differentiation was maximally inhibited by HIV-1 and LAV-2 when these proteins were added concurrently to cultures with the polyclonal B cell activators pokeweed mitogen or Epstein-Barr virus. Tetanus antigen-specific T cell lymphoproliferation was inhibited by all retroviral proteins. These findings suggest that related retroviruses differ in their capacity to influence normal immune responses.
人类免疫缺陷病毒（HIV-1）具有严重的免疫抑制作用[Spickett和Dalgleish（1988年），《临床与实验免疫学》71卷，第1期]。在本通讯中，我们研究了HIV-1（BH10）、HIV-2（LAV-2）和STLV-3对健康志愿者B细胞和T细胞的影响。发现B淋巴细胞在受到HIV-1和LAV-2蛋白刺激时可分化为免疫球蛋白分泌细胞，但对STLV-3蛋白无反应。这种反应在蛋白浓度为0.05 - 0.005微克/毫升时出现，且依赖于T细胞。在EB病毒转化的B细胞系SKW 6.4中，仅HIV-1可诱导IgM分泌。在较高浓度下，所有三种逆转录病毒制剂对B细胞和T淋巴细胞的功能均有抑制作用。当这些蛋白与多克隆B细胞激活剂商陆有丝分裂原或爱泼斯坦 - 巴尔病毒同时添加到培养物中时，HIV-1和LAV-2对B细胞分化的抑制作用最大。所有逆转录病毒蛋白均抑制破伤风抗原特异性T细胞增殖。这些发现表明，相关逆转录病毒影响正常免疫反应的能力有所不同。
