National Institute of Animal Nutrition and Physiology, Adugodi, Bangalore, Karnataka, 560 030, India.
Sci Rep. 2022 Jul 1;12(1):11170. doi: 10.1038/s41598-022-15211-9.
A laccase-producing hyper performer, Schizophyllum commune, a white-rot fungus, was evaluated for its ability to selectively degrade lignin of diverse crop residues in vitro. Relative analysis of crop residue treatment using laccase obtained from immobilized cells demonstrated degradation of 30-40% in finger millet straw and sorghum stover, 27-32% in paddy straw, 21% in wheat straw, and 26% in maize straw, while 20% lignin degradation was observed when purified and recombinant laccase was used. Further investigations into in vitro dry matter digestibility studies gave promising results recording digestibility of 54-59% in finger millet straw 33-36% in paddy straw and wheat straw, 16% in maize straw for laccase obtained from cell immobilization method, whereas 14% digestibility was observed when purified and recombinant laccase was used. Sorghum stover recorded digestibility of 13-15% across all straws treated with laccase. The results obtained elucidated the positive influence of laccase treatment on lignin degradation and in vitro dry matter digestibility. The present research gave encouraging figures confirming the production of laccase using the cell immobilization method to be an efficient production method commensurate with purified and recombinant laccase under conditions of submerged cultivation, proclaiming a cost-effective, environmentally safe green technology for effectual lignin depolymerization.
一种产漆酶的高效菌株，栓菌（Schizophyllum commune），是一种白腐真菌，被评估其在体外选择性降解不同作物秸秆木质素的能力。使用固定化细胞获得的漆酶对作物秸秆处理的相对分析表明，在小米秸秆和高粱秸秆中降解了 30-40%，在水稻秸秆中降解了 27-32%，在小麦秸秆中降解了 21%，在玉米秸秆中降解了 26%，而使用纯化和重组漆酶时观察到 20%的木质素降解。进一步进行体外干物质消化率研究得到了有希望的结果，记录到用细胞固定化方法获得的漆酶在小米秸秆中的消化率为 54-59%，在水稻秸秆和小麦秸秆中的消化率为 33-36%，在玉米秸秆中的消化率为 16%，而使用纯化和重组漆酶时观察到 14%的消化率。高粱秸秆在所有用漆酶处理的秸秆中记录的消化率为 13-15%。研究结果阐明了漆酶处理对木质素降解和体外干物质消化率的积极影响。本研究给出了令人鼓舞的数字，证实了使用细胞固定化方法生产漆酶是一种有效的生产方法，与在浸没培养条件下使用纯化和重组漆酶相当，宣称了一种具有成本效益、环境安全的绿色技术，可有效地进行木质素解聚。
