Centre of Bioanalysis, National Institute of Research and Development for Biological Sciences, Bucharest, 296 Splaiul Independentei, 060031 Bucharest, Romania.
Centre of Bioanalysis, National Institute of Research and Development for Biological Sciences, Bucharest, 296 Splaiul Independentei, 060031 Bucharest, Romania.
Biosens Bioelectron. 2016 Jan 15;75:232-7. doi: 10.1016/j.bios.2015.08.051. Epub 2015 Aug 22.
A nanocomposite formed from molybdenum disulphide (MoS2) and graphene quantum dots (GQDs) was proposed as a novel and suitable support for enzyme immobilisation displaying interesting electrochemical properties. The conductivity of the carbon based screen-printed electrodes was highly improved after modification with MoS2 nanoflakes and GQDs, the nanocomposite also providing compatible matrix for laccase immobilisation. The influence of different modification steps on the final electroanalytical performances of the modified electrode were evaluated by UV-vis absorption and fluorescence spectroscopy, scanning electron microscopy, transmission electron microscopy, X ray diffraction, electrochemical impedance spectroscopy and cyclic voltammetry. The developed laccase biosensor has responded efficiently to caffeic acid over a concentration range of 0.38-100µM, had a detection limit of 0.32µM and a sensitivity of 17.92nAµM(-1). The proposed analytical tool was successfully applied for the determination of total polyphenolic content from red wine samples.
一种由二硫化钼（MoS2）和石墨烯量子点（GQDs）组成的纳米复合材料被提议作为一种新型的、合适的酶固定化载体，具有有趣的电化学性质。经过 MoS2 纳米片和 GQDs 的修饰，碳基丝网印刷电极的导电性得到了极大的提高，纳米复合材料也为漆酶的固定化提供了相容的基质。通过紫外-可见吸收光谱、荧光光谱、扫描电子显微镜、透射电子显微镜、X 射线衍射、电化学阻抗谱和循环伏安法评估了不同修饰步骤对修饰电极最终电分析性能的影响。所开发的漆酶生物传感器对咖啡酸在 0.38-100µM 的浓度范围内有效响应，检测限为 0.32µM，灵敏度为 17.92nAµM(-1)。该分析工具成功应用于红酒样品中总多酚含量的测定。
