Central Scientific Instruments Organisation (CSIR-CSIO), Sector 30-C, Chandigarh, 160030, India; Academy of Scientific and Innovative Research, CSIR-CSIO, Sector 30-C, Chandigarh, 160030, India; CSIR-Institute of Microbial Technology (CSIR-IMTECH), Sector 39, Chandigarh, 160038, India.
Department of Chemistry, Hanyang University, Seoul, 133-791, Republic of Korea.
Biosens Bioelectron. 2016 Dec 15;86:548-556. doi: 10.1016/j.bios.2016.07.052. Epub 2016 Jul 17.
A label-free immunosensor based on electrochemical impedance spectroscopy has been developed for the sensitive detection of a cardiac biomarker myoglobin (cMyo). Hydrothermally synthesized graphene quantum dots (GQDs) have been used as an immobilized template on screen printed electrodes for the construction of an impedimetric sensor platform. The GQDs-modified electrode was conjugated with highly specific anti-myoglobin antibodies to develop the desired immunosensor. The values of charge transfer resistance (Rct) were monitored as a function of varying antigen concentration. The Rct value of the immunosensor showed a linear increase (from 0.20 to 0.31kΩ) in the range of 0.01-100ng/mL cMyo. The specific detection of cMyo was also made in the presence of other competing proteins. The limit of detection for the proposed immunosensor was estimated as 0.01ng/mL which is comparable to the standard ELISA techniques.
一种基于电化学阻抗谱的无标记免疫传感器已被开发出来，用于灵敏检测心脏标志物肌红蛋白（cMyo）。水热合成的石墨烯量子点（GQDs）被用作在丝网印刷电极上的固定模板，以构建阻抗传感器平台。GQDs 修饰的电极与高度特异性的抗肌红蛋白抗体结合，以开发所需的免疫传感器。作为抗原浓度变化的函数，监测了电荷转移电阻（Rct）的值。免疫传感器的 Rct 值在 0.01-100ng/mL cMyo 的范围内呈线性增加（从 0.20 增加到 0.31kΩ）。在存在其他竞争蛋白的情况下，也对 cMyo 进行了特异性检测。所提出的免疫传感器的检测限估计为 0.01ng/mL，可与标准 ELISA 技术相媲美。
