Roy Pascalin, Nadeau Mélanie, Valle Marion, Bellmann Kerstin, Marette André, Tchernof André, Gagnon Claudia
Department of Medicine, Laval University, 1050, de la Médecine avenue, Québec G1V 0A6, Canada.
Department of Medicine, Laval University, 1050, de la Médecine avenue, Québec G1V 0A6, Canada; Obesity and Metabolism Unit, Quebec Heart and Lung Institute Research Centre, 2725, Sainte-Foy Road, Québec G1V 4G5, Canada; Endocrinology and Nephrology Unit, CHU de Québec Research Centre, 2705, Laurier Boulevard, Québec G1V 4G2, Canada.
Steroids. 2015 Dec;104:65-71. doi: 10.1016/j.steroids.2015.08.014. Epub 2015 Aug 28.
Both vitamin D deficiency and inflammation have been associated with insulin resistance and type 2 diabetes risk. In vitro vitamin D treatment of subcutaneous (SC) adipose tissue (AT) may reduce inflammation, but data are conflicting.
To evaluate the effects of vitamin D (25(OH)D3 and 1,25(OH)2D3) on the secretion of inflammatory cytokines (TNF-α and IL-6) in omental (OM) and SC human AT and to explore factors that could correlate with the individual response to vitamin D including age, smoking status, BMI, comorbidities, medication, HbA1c, apolipoprotein B, serum 25-hydroxyvitamin D and high sensitivity C-reactive protein.
7 men and 8 women with severe obesity undergoing bariatric surgery.
Fresh OM and SC AT explants sampled during surgery (n=15) were incubated for 24h in a control, 25(OH)D3 (150 nM) or 1,25(OH)2D3 (1 nM) medium. Lipopolysaccharide (LPS) (10 ng/ml) was added for another 24h.
Change in TNF-α and IL-6 levels in collected media after vitamin D treatment (ELISA).
Mean age and BMI of the patients were 46.4±10.9 years and 48.8±7.5 kg/m(2), respectively. Eleven patients had type 2 diabetes. 25(OH)D3 and 1,25(OH)2D3 reduced the LPS-induced increases in cytokine levels in OM AT of women but not in men. No effect was observed in SC AT. Apart from gender, none of the factors analyzed correlated with vitamin D response.
We showed that 25(OH)D3 and 1,25(OH)2D3 can lower cytokine release from OM but not SC AT explants and only in women.
维生素D缺乏和炎症均与胰岛素抵抗及2型糖尿病风险相关。体外对皮下（SC）脂肪组织（AT）进行维生素D处理可能会减轻炎症，但数据存在矛盾。
评估维生素D（25(OH)D3和1,25(OH)2D3）对网膜（OM）和人皮下脂肪组织中炎性细胞因子（TNF-α和IL-6）分泌的影响，并探索可能与个体对维生素D反应相关的因素，包括年龄、吸烟状况、体重指数、合并症、用药情况、糖化血红蛋白、载脂蛋白B、血清25-羟基维生素D和高敏C反应蛋白。
7名男性和8名女性重度肥胖患者接受减肥手术。
手术期间采集的新鲜网膜和皮下脂肪组织外植体（n = 15）在对照培养基、25(OH)D3（150 nM）或1,25(OH)2D3（1 nM）培养基中孵育24小时。再加入脂多糖（LPS）（10 ng/ml）孵育24小时。
维生素D处理后收集的培养基中TNF-α和IL-6水平的变化（酶联免疫吸附测定）。
患者的平均年龄和体重指数分别为46.4±10.9岁和48.8±7.5 kg/m²。11名患者患有2型糖尿病。25(OH)D3和1,25(OH)2D3可降低LPS诱导的女性网膜脂肪组织中细胞因子水平的升高，但对男性无效。在皮下脂肪组织中未观察到效果。除性别外，分析的其他因素均与维生素D反应无关。
我们发现25(OH)D3和1,25(OH)2D3可降低网膜而非皮下脂肪组织外植体中的细胞因子释放，且仅在女性中有效。
