Murate Motohide, Kobayashi Toshihide
Lipid Biology Laboratory, RIKEN, Wako, Saitama 351-0198, Japan.
Lipid Biology Laboratory, RIKEN, Wako, Saitama 351-0198, Japan; Institut National de la Santé et de la Recherche Médicale (INSERM) Unité 1060, Université Lyon 1, Villeurbanne, 69621, France.
Chem Phys Lipids. 2016 Jan;194:58-71. doi: 10.1016/j.chemphyslip.2015.08.009. Epub 2015 Aug 28.
Whereas asymmetric transbilayer lipid distribution in the plasma membrane is well recognized, methods to examine the precise localization of lipids are limited. In this review, we critically evaluate the methods that are applied to study transbilayer asymmetry of lipids, summarizing the factors that influence the measurement. Although none of the present methods is perfect, the current application of immunoelectron microscopy-based technique provides a new picture of lipid asymmetry. Next, we summarize the transbilayer distribution of individual lipid in both erythrocytes and nucleated cells. Finally we discuss the concept of the interbilayer communication of lipids.
尽管质膜中不对称的跨膜脂质分布已得到充分认识，但用于检测脂质精确定位的方法却很有限。在本综述中，我们批判性地评估了用于研究脂质跨膜不对称性的方法，总结了影响测量的因素。虽然目前没有一种方法是完美的，但基于免疫电子显微镜技术的当前应用为脂质不对称性提供了新的认识。接下来，我们总结了红细胞和有核细胞中单个脂质的跨膜分布。最后，我们讨论了脂质双层间通讯的概念。
