Di Cristanziano Veronica, Timmen-Wego Monika, Lübke Nadine, Kaiser Rolf, Pfister Herbert, Di Cave David, Berrilli Federica, Kaboré Yolande, D'Alfonso Rossella
University of Cologne, Cologne, Germany.
J Infect Dev Ctries. 2015 Aug 29;9(8):884-9. doi: 10.3855/jidc.6460.
Gastrointestinal infections caused by viruses, bacteria, and parasites are endemic in most developing countries due to inadequate provision of safe water supplies, sanitation, and hygiene. To investigate the enteric pathogens infecting people living in Côte d'Ivoire, the Luminex Gastrointestinal Pathogen Panel (xTAG GPP) assay was used to analyze 34 human fecal samples. This study represents the first application of this technology to samples from a sub-Saharan African country.
Thirty-four stool samples from asymptomatic and symptomatic patients, 1-15 years of age, were analyzed by xTAG GPP. The Luminex assay represents a qualitative bead-based multiplexed molecular diagnostic test able to identify concurrently 15 enteric pathogens, including bacteria, viruses, and parasites.
Overall, 22 out of 34 (64.7%) fecal specimens were detected to be positive by xTAG GPP. Sixteen were from asymptomatic subjects, and 10 patients (45.4%) showed co-infections. G. duodenalis was detected in 15 patients, in both mono- and co-infections, representing the most frequent pathogen, followed by enterotoxigenic Escherichia coli (ETEC) LT/ST. Four norovirus isolates were also detected and assigned to genogroups I and II.
Considering the burden of enteric infections in developing countries, particularly among children, and the high rate of co-infections in asymptomatic subjects, this study shows the need for diagnostic tools such as xTAG GPP to improve diagnosis and treatment of these infections in endemic areas.
由于安全供水、卫生设施和个人卫生条件不足，病毒、细菌和寄生虫引起的胃肠道感染在大多数发展中国家呈地方流行态势。为调查感染科特迪瓦人民的肠道病原体，采用Luminex胃肠道病原体检测板（xTAG GPP）分析法对34份人类粪便样本进行分析。本研究是该技术首次应用于撒哈拉以南非洲国家的样本。
采用xTAG GPP分析法对34份1至15岁无症状和有症状患者的粪便样本进行分析。Luminex分析法是一种基于微珠的定性多重分子诊断测试，能够同时识别15种肠道病原体，包括细菌、病毒和寄生虫。
总体而言，34份粪便标本中有22份（64.7%）通过xTAG GPP检测呈阳性。16份来自无症状受试者，10名患者（45.4%）出现合并感染。15名患者检测出十二指肠贾第虫，包括单一感染和合并感染，是最常见的病原体，其次是产肠毒素大肠杆菌（ETEC）LT/ST。还检测到4株诺如病毒分离株，并分为基因群I和II。
考虑到发展中国家肠道感染的负担，特别是儿童中的负担，以及无症状受试者中合并感染的高发生率，本研究表明需要xTAG GPP等诊断工具来改善流行地区这些感染的诊断和治疗。
