Khin Nwe O o, Sebastian A A, Aye T
J Diarrhoeal Dis Res. 1989 Sep-Dec;7(3-4):81-4.
The carriage of enteric bacterial pathogens by house flies, caught from different places of human habitation and at different seasons, was quantitatively determined in the town of Tamwe, Yangon, Myanmar. A total of 186 fly-pools, each containing 10 live flies, were caught in entomological cages by random sampling regarding season, month, frequency and place of collection. A 10% suspension of each homogenised fly-pool in sterile normal saline was prepared on the day of collection and was cultured to isolate pathogens using standard microbiological methods. Escherichia coli, Vibrio cholerae non-O1, and species of Salmonella and Shigella were isolated from 76.3%, 45.7%, 11.8% and 4.8% respectively from all the fly-pools. Enteric bacterial pathogens were isolated more frequently in flies from refuse dumps and animal pens and least commonly in flies from kitchens. Of the E. coli isolates, one pool each from flies trapped in animal pens (n = 30 fly-pools), latrines (n = 32) and refuse dumps (n = 34) produced enterotoxins (ETEC). These ETEC isolates were obtained in the months of June and July. The rates of isolation of pathogens were highest in the hot-wet season. The faecal coliform counts in the fly-pools also increased in this season compared with those in cool-dry and hot-dry seasons. These data suggest that flies may be an important carrier of enteric infections in Myanmar.
在缅甸仰光的丹兑镇，对从人类居住的不同地点和不同季节捕获的家蝇携带肠道细菌病原体的情况进行了定量测定。通过对季节、月份、采集频率和地点进行随机抽样，在昆虫笼中总共捕获了186个蝇池，每个蝇池包含10只活蝇。在采集当天，将每个匀浆后的蝇池制成10%的无菌生理盐水悬液，并采用标准微生物学方法进行培养以分离病原体。在所有蝇池中，分别从76.3%、45.7%、11.8%和4.8%的样本中分离出大肠杆菌、非O1群霍乱弧菌、沙门氏菌属和志贺氏菌属。在垃圾场和畜栏捕获的苍蝇中，肠道细菌病原体的分离频率更高，而在厨房捕获的苍蝇中分离频率最低。在分离出的大肠杆菌中，从畜栏（n = 30个蝇池）、厕所（n = 32）和垃圾场（n = 34）捕获的苍蝇中各有一个蝇池产生了肠毒素（产肠毒素大肠杆菌）。这些产肠毒素大肠杆菌分离株是在6月和7月获得的。病原体的分离率在湿热季节最高。与凉爽干燥和炎热干燥季节相比，该季节蝇池中的粪大肠菌群计数也有所增加。这些数据表明，苍蝇可能是缅甸肠道感染的重要传播媒介。
