a Department of Biology, Science Faculty , Selcuk University , Konya , Turkey .
b Department of Biology, Science and Arts Faculty , Erzincan University , Erzincan , Turkey .
J Enzyme Inhib Med Chem. 2016 Dec;31(6):983-90. doi: 10.3109/14756366.2015.1077822. Epub 2015 Aug 31.
We investigated the antioxidant and enzyme inhibitory activities and chemical composition of the hydro-distilled essential oil (0.35% yield) from aerial parts of Thymus spathulifolius. Antioxidant capacity of the oil was assessed by different methods including free radical scavenging (DPPH and ABTS), reducing power (FRAP and CUPRAC) and phosphomolybdenum assay. Inhibitory activities were analyzed against acetylcholinesterase (AChE), butyrylcholinesterase (BChE), α-amylase, α-glucosidase, and tyrosinase. Twenty-one constituents were identified representing 97.2% of the total oil with thymol (50.5%), borneol (16.7%) and carvacrol (7.7%) as the major components. The essential oil exhibited good antioxidant activity with IC50 values of 3.82 and 0.22 mg/mL determined by free radical scavenging DPPH and ABTS, respectively. EC50 values of FRAP and CUPRAC were found to be 0.12 and 0.34 mg/mL, respectively. The results of the present study support the uses of T. spathulifolius essential oil as a source of natural antioxidants and bioactivities for functional foods and phytomedicines.
我们研究了狭叶薰衣草地上部分水蒸馏得到的精油（产率为 0.35%）的抗氧化和酶抑制活性及化学成分。通过不同方法评估了油的抗氧化能力，包括自由基清除（DPPH 和 ABTS）、还原能力（FRAP 和 CUPRAC）和磷钼酸盐测定。分析了其对乙酰胆碱酯酶（AChE）、丁酰胆碱酯酶（BChE）、α-淀粉酶、α-葡萄糖苷酶和酪氨酸酶的抑制活性。鉴定了 21 种成分，占总油量的 97.2%，其中主要成分为百里酚（50.5%）、龙脑（16.7%）和香芹酚（7.7%）。该精油具有良好的抗氧化活性，自由基清除 DPPH 和 ABTS 的 IC50 值分别为 3.82 和 0.22mg/mL。FRAP 和 CUPRAC 的 EC50 值分别为 0.12 和 0.34mg/mL。本研究结果支持将狭叶薰衣草精油用作天然抗氧化剂和功能性食品和植物药生物活性的来源。
