Sevindik Handan Gokben, Ozgen Ufuk, Atila Alptug, Ozturk Er Handan, Kazaz Cavit, Duman Hayri
Faculty of Pharmacy, Department of Pharmacognosy Atatürk University.
Chem Pharm Bull (Tokyo). 2015;63(9):720-5. doi: 10.1248/cpb.c14-00877.
Thymus praecox ssp. grossheimii (RONNIGER) JALAS var. grossheimii (Lamiaceae) is used as an herbal tea for cold, stomachache, cough, and infections in Turkey. There are no phytochemical studies on this species. We performed phytochemical studies and quantitative analysis of rosmarinic acid and luteolin 5-O-β-D-glucopyranoside in the methanol extract of the plant. Several chromatographic methods were used for the isolation of major compounds. HPLC methods were applied for quantitative analysis of rosmarinic acid and luteolin 5-O-β-D-glucopyranoside in the methanol extract. In this study, ursolic acid (1), oleanolic acid (2), methyl rosmarinate (3), ethyl rosmarinate (4), rosmarinic acid (5), luteolin 5-O-β-D-glucopyranoside (6), and thymoquinol 2,5-O-β-diglucopyranoside (7) were isolated from the aerial parts of the plant. The relative contents of rosmarinic acid and luteolin 5-O-β-D-glucopyranoside in the extract were 15.2 and 57.8 mg/g of dry weight, respectively. Compounds isolated from this plant and the contents of rosmarinic acid and luteolin 5-O-β-D-glucopyranoside provided reasonable evidence for the traditional usages of this plant.
早花百里香草原变种粗枝百里香（唇形科）在土耳其被用作治疗感冒、胃痛、咳嗽和感染的草药茶。目前尚未对该物种进行植物化学研究。我们对该植物甲醇提取物中的迷迭香酸和木犀草素5-O-β-D-葡萄糖苷进行了植物化学研究和定量分析。采用了几种色谱方法来分离主要化合物。应用高效液相色谱法对甲醇提取物中的迷迭香酸和木犀草素5-O-β-D-葡萄糖苷进行定量分析。在本研究中，从该植物地上部分分离出了熊果酸（1）、齐墩果酸（2）、迷迭香酸甲酯（3）、迷迭香酸乙酯（4）、迷迭香酸（5）、木犀草素5-O-β-D-葡萄糖苷（6）和百里醌2,5-O-β-二葡萄糖苷（7）。提取物中迷迭香酸和木犀草素5-O-β-D-葡萄糖苷的相对含量分别为15.2和57.8mg/g干重。从该植物中分离出的化合物以及迷迭香酸和木犀草素5-O-β-D-葡萄糖苷的含量为该植物的传统用途提供了合理的证据。
