Division of Health Sciences, Department of Clinical Nursing, Graduate Course of Nursing Science, Graduate School of Medical Sciences, Kanazawa University, Ishikawa, Japan.
Department of Medical Laboratory Science, Faculty of Nursing and Health Sciences, Universitas Muhammadiyah Semarang, Semarang, Indonesia.
PLoS One. 2022 Oct 14;17(10):e0275602. doi: 10.1371/journal.pone.0275602. eCollection 2022.
A non-thermal atmospheric pressure plasma jet (APPJ) may stimulate cells and tissues or result in cell death depending on the intensity of plasma at the target; therefore, we herein investigated the effects of non-thermal plasma under non-contact conditions on the healing of full-thickness wounds in diabetic mice (DM+ group) and normal mice (DM- group). A hydrogen peroxide colorimetric method and high performance liquid chromatography showed that APPJ produced low amounts of reactive oxygen and nitrogen species. Ten-week-old male C57BL/6j mice with normal blood glucose levels (DM- group) and 10-week-old male C57BLKS/J Iar-+Leprdb/+Leprdb mice (DM+ group) received two full-thickness cutaneous wounds (4 mm in diameter) on both sides of the dorsum. Wounds were treated with or without the plasma jet or argon gas for 1 minute and were then covered with a hydrocolloid dressing (Hydrocolloid), according to which mice were divided into the following groups: DM+Plasma, DM+Argon, DM+Hydrocolloid, DM-Plasma, DM-Argon, and DM-Hydrocolloid. Exudate weights, wound areas, and wound area ratios were recorded every day. Hematoxylin and eosin staining was performed to assess re-epithelialization and α-SMA immunohistological staining to evaluate the formation of new blood vessels. Non-thermal plasma under non-contact conditions reduced the production of exudate. Exudate weights were smaller in the DM+Plasma group than in the DM+Hydrocolloid and DM+Argon groups. The wound area ratio was smaller for plasma-treated wounds, and was also smaller in the DM+Plasma group than in the DM+Hydrocolloid and DM+Argon groups on days 1-21 (p<0.01). Wound areas were smaller in the DM-Plasma group than in the DM-Argon group until day 14 and differences were significant on days 1-5 (p<0.01). The percentage of re-epithelialization was significantly higher in the DM+Plasma group than in the DM+Argon and DM+Hydrocolloid groups (p<0.01). The number of new blood vessels that had formed by day 7 was significantly higher in the DM+Plasma group than in the DM+Hydrocolloid and DM+Argon groups (p<0.05). These results indicate that treatment with the current non-thermal plasma APPJ device under non-contact conditions accelerated wound healing in diabetic mice.
非热常压等离子体射流（APPJ）可能会刺激细胞和组织，或者导致细胞死亡，这取决于目标处等离子体的强度；因此，我们在此研究了非接触条件下非热等离子体对糖尿病小鼠（DM+组）和正常小鼠（DM-组）全层伤口愈合的影响。过氧化氢比色法和高效液相色谱法显示，APPJ 产生的活性氧和氮物种含量较低。10 周龄雄性 C57BL/6j 血糖水平正常的小鼠（DM-组）和 10 周龄雄性 C57BLKS/J Iar-+Leprdb/+Leprdb 小鼠（DM+组）在背部两侧接受两个直径为 4 毫米的全层皮肤伤口。伤口用等离子射流或氩气处理 1 分钟，然后用水胶体敷料（Hydrocolloid）覆盖，根据此方法将小鼠分为以下几组：DM+Plasma、DM+Argon、DM+Hydrocolloid、DM-Plasma、DM-Argon 和 DM-Hydrocolloid。每天记录渗出物重量、伤口面积和伤口面积比。进行苏木精和伊红染色以评估再上皮化，并用 α-SMA 免疫组织化学染色评估新血管的形成。非接触条件下的非热等离子体减少了渗出物的产生。与 DM+Hydrocolloid 和 DM+Argon 组相比，DM+Plasma 组的渗出物重量更小。处理过的伤口的伤口面积比更小，在第 1-21 天，与 DM+Hydrocolloid 和 DM+Argon 组相比，DM+Plasma 组的伤口面积比也更小（p<0.01）。在第 1-14 天，DM-Plasma 组的伤口面积小于 DM-Argon 组，差异在第 1-5 天有统计学意义（p<0.01）。与 DM+Argon 和 DM+Hydrocolloid 组相比，DM+Plasma 组的再上皮化百分比显著更高（p<0.01）。第 7 天形成的新血管数量在 DM+Plasma 组显著高于 DM+Hydrocolloid 和 DM+Argon 组（p<0.05）。这些结果表明，在非接触条件下使用当前的非热常压等离子体 APPJ 装置治疗可加速糖尿病小鼠的伤口愈合。
